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dc.contributor.advisorGreenlief, Michaeleng
dc.contributor.authorWan, Lili, 1976-eng
dc.date.issued2007eng
dc.date.submitted2007 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on March 4, 2008)eng
dc.descriptionVita.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2007.eng
dc.description.abstractThe primary goal of this dissertation research was to develop methods for routine speciation analysis of selenium in tissues. A method for total selenium using on-line stable isotope dilution analysis with conventional ICP-MS (SIDA-ICP-MS) was developed. Masses at 77, 78, 79, 81, and 82 were monitored and quantitation of Se was determined based on both 78Se and 82Se. SIDA-ICP-MS was successfully applied to the determination of total selenium in biological materials, such as selenized yeasts, certified reference materials, lab-cultured oligochaetes and desert pupfish. The separation and quantitation of seleno-amino acids was accomplished by ion-pairing reversed-phase liquid chromatography (RPLC) and detected by ICP-MS at mass 82 using the standard mode. It was found that methanesulfonic acidic hydrolysis demonstrated a higher extraction efficiency of selenomethionine than enzymatic digestion. Selenomethionine (SeMet) was the only significant Se-containing species detected in the biological samples that were examined.eng
dc.identifier.merlinb62412942eng
dc.identifier.oclc212783755eng
dc.identifier.urihttps://hdl.handle.net/10355/4683
dc.identifier.urihttps://doi.org/10.32469/10355/4683eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subjectselenomethionine; selenized yeasts.eng
dc.subjectselenomethionine; selenized yeastseng
dc.subject.lcshSelenium -- Speciationeng
dc.subject.lcshYeasteng
dc.subject.lcshOligochaetaeng
dc.subject.lcshDesert pupfisheng
dc.subject.lcshChromatographic analysiseng
dc.titleDetermination of total selenium and seleno-amino acids in yeast and aquatic organisms by liquid chromatography and inductively coupled plasma mass spectrometryeng
dc.typeThesiseng
thesis.degree.disciplineChemistry (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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