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    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2007 Dissertations (MU)
    • 2007 MU dissertations - Freely available online
    • View Item
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    Quantitative biological studies at cellular and sub-cellular level

    Hosu, Basarab Gabriel, 1968-
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    [PDF] research.pdf (7.870Mb)
    Date
    2007
    Format
    Thesis
    Metadata
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    Abstract
    We developed a translational magnetic tweezers for quantitative measurements at cellular and sub-cellular level. In the cellular studies, multiple membrane tethers were extracted simultaneously under constant force transduced to eukaryotic cells through magnetic beads attached to their membranes. The tethers were characterized in terms of viscoelastic parameters. The contribution of the actin cytoskeleton in the process of tether formation was investigated. The membrane tether system was used to test the applicability of the Crooks fluctuation theorem (a recent finding in non-equilibrium thermodynamics) at the mesoscopic level. In the sub-cellular studies, the cytoplasmic viscoelastic coefficients of mouse oocytes were determined using magnetic beads trapped into their cytoskeletal mesh. We found that cryopreservation altered all the viscoelastic parameters. We demonstrated that the reversible disassembly of the actin cytoskeleton with latrunculin A before cryopreservation increased the number of survivors and preserved their viscoelastic parameters. This finding promoted latrunculin as a candidate cryoprotective agent.
    URI
    https://hdl.handle.net/10355/4689
    https://doi.org/10.32469/10355/4689
    Degree
    Ph. D.
    Thesis Department
    Biological sciences (MU)
    Collections
    • Biological Sciences electronic theses and disserations (MU)
    • 2007 MU dissertations - Freely available online

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