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dc.contributor.advisorPintel, David J.eng
dc.contributor.authorYe, Chaoyang, 1979-eng
dc.date.issued2007eng
dc.date.submitted2007 Springeng
dc.description"May 2007"eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionVita.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2007.eng
dc.description.abstractThe small genome size of adeno-associated viruses demand efficient gene regulation achieved by various strategies. This research compared these strategies from AAV2, AAV5 and bovine-AAV, and found that AAV2 capsid promoter P40 has a low basal level and is activated by its non-structural Rep in the presence of adenovirus while AAV5 capsid promoter P41 has a higher basal level and is not further activated by Rep. The difference of the activation ability between AAV2 and AAV5 Rep resides in the amino terminus of the protein. The higher basal level of P41 is at least partially contributed to the AP1 and CRE transcription factor binding sites upstream. Although BAAV, a closer relative to AAV5, has both sites, its capsid promoter activity is still low and can be further activated by both its own Rep and AAV5 Rep. This study not only enriched our understanding of the basic molecular biology of these viruses, which potentially would benefit gene therapy vector design, but also provided clue of the evolution migration between the animal and human AAVs.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.identifier.merlinb5929162xeng
dc.identifier.oclc163599160eng
dc.identifier.urihttps://doi.org/10.32469/10355/4709eng
dc.identifier.urihttps://hdl.handle.net/10355/4709
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subject.lcshGenetic regulationeng
dc.subject.lcshParvoviruseseng
dc.subject.lcshViral proteins -- Physiologyeng
dc.subject.lcshGenetic transcriptioneng
dc.subject.meshDependoviruseng
dc.subject.meshTranscription, Geneticeng
dc.subject.meshGene Expression Regulation, Viraleng
dc.subject.meshViral Proteins -- physiologyeng
dc.titleTranscription regulation of adeno-associated viruseseng
dc.typeThesiseng
thesis.degree.disciplineMicrobiology (Medicine) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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