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    • Graduate School - MU Theses and Dissertations (MU)
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    • 2007 Dissertations (MU)
    • 2007 MU dissertations - Freely available online
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    Nano- and micro-scale studies of exocytosis

    Shin, Won-Chul, 1973-
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    Date
    2007
    Format
    Thesis
    Metadata
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    Abstract
    Neurons and neuroendocrine cells contain vesicles packed with hormones or neurotransmitters. Upon appropriate stimulation, a rise in intracellular Ca2+ concentration triggers the fusion of vesicles with the outer membrane of cells and release of vesicle contents into the extracellular space in a process called exocytosis. In this thesis, we developed three new nano- and micro- techniques to study exocytosis. 1. We used scanning ion conductance microscopy (SICM) to image changes in the surface membrane of adrenal chromaffin cells after stimulation of exocytosis. Punctate depressions were noted in clusters of two or more. Increases in membrane surface area, consistent with the fusion and collapse of one or more vesicles into the surface membrane, were observed 64% of the cells. 2. We used a microcontact printing method with PDMS stamps by "soft" lithography to pattern microislands of rat hippocampal neurons to form autapses. Neurons on microstamped microislands survived and grew neurites for more than 21 days and resembled microisland cultures formed by the traditional method of spraying collagen on agarose coated substrates. 3. Microfabricated devices were developed to electrochemically measure quantal catecholamine release from an array of individual cells. Here we report patterning of cellsized holes in [tilde operator] 15 m-thick films. These films are placed on transparent indium tin oxide electrodes to insulate the unused part of the electrode whereas the holes in the film both determine the location of the working electrode and serve as pockets for cell trapping. We found that this approach represents a simple and effective way to target cells to electrodes to record amperometric spikes.
    URI
    https://doi.org/10.32469/10355/4725
    https://hdl.handle.net/10355/4725
    Degree
    Ph. D.
    Thesis Department
    Biological engineering (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • 2007 MU dissertations - Freely available online
    • Biological Engineering electronic theses and dissertations - CAFNR (MU)
    • Biological Engineering electronic theses and dissertations - Engineering (MU)

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