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dc.contributor.advisorGillis, Kevin D.en
dc.contributor.authorShin, Won-Chul, 1973-en_US
dc.date.issued2007eng
dc.date.submitted2007 Springen
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from title screen of research.pdf file (viewed on March 24, 2009)en_US
dc.descriptionVita.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionThesis (Ph.D.) University of Missouri-Columbia 2007.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.en_US
dc.description.abstractNeurons and neuroendocrine cells contain vesicles packed with hormones or neurotransmitters. Upon appropriate stimulation, a rise in intracellular Ca2+ concentration triggers the fusion of vesicles with the outer membrane of cells and release of vesicle contents into the extracellular space in a process called exocytosis. In this thesis, we developed three new nano- and micro- techniques to study exocytosis. 1. We used scanning ion conductance microscopy (SICM) to image changes in the surface membrane of adrenal chromaffin cells after stimulation of exocytosis. Punctate depressions were noted in clusters of two or more. Increases in membrane surface area, consistent with the fusion and collapse of one or more vesicles into the surface membrane, were observed 64% of the cells. 2. We used a microcontact printing method with PDMS stamps by "soft" lithography to pattern microislands of rat hippocampal neurons to form autapses. Neurons on microstamped microislands survived and grew neurites for more than 21 days and resembled microisland cultures formed by the traditional method of spraying collagen on agarose coated substrates. 3. Microfabricated devices were developed to electrochemically measure quantal catecholamine release from an array of individual cells. Here we report patterning of cellsized holes in [tilde operator] 15 m-thick films. These films are placed on transparent indium tin oxide electrodes to insulate the unused part of the electrode whereas the holes in the film both determine the location of the working electrode and serve as pockets for cell trapping. We found that this approach represents a simple and effective way to target cells to electrodes to record amperometric spikes.en_US
dc.identifier.merlin.b66671358en_US
dc.identifier.oclc316861666en_US
dc.identifier.otherShinW-031609-D6333en_US
dc.identifier.urihttp://hdl.handle.net/10355/4725
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2007 Freely available dissertations (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2007 Dissertations
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshExocytosisen_US
dc.subject.lcshConductometric analysisen_US
dc.subject.lcshNeuronsen_US
dc.subject.lcshParaneuronsen_US
dc.titleNano- and micro-scale studies of exocytosisen_US
dc.typeThesisen_US
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh.D.eng


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