Nano- and micro-scale studies of exocytosis

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Nano- and micro-scale studies of exocytosis

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/4725

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dc.contributor.advisor Gillis, Kevin D. en
dc.contributor.author Shin, Won-Chul, 1973- en_US
dc.date.accessioned 2010-01-12T18:41:11Z
dc.date.available 2010-01-12T18:41:11Z
dc.date.issued 2007 en_US
dc.date.submitted 2007 Spring en
dc.identifier.other ShinW-031609-D6333 en_US
dc.identifier.uri http://hdl.handle.net/10355/4725
dc.description The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. en_US
dc.description Title from title screen of research.pdf file (viewed on March 24, 2009) en_US
dc.description Vita. en_US
dc.description Includes bibliographical references. en_US
dc.description Thesis (Ph.D.) University of Missouri-Columbia 2007. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Biological engineering. en_US
dc.description.abstract Neurons and neuroendocrine cells contain vesicles packed with hormones or neurotransmitters. Upon appropriate stimulation, a rise in intracellular Ca2+ concentration triggers the fusion of vesicles with the outer membrane of cells and release of vesicle contents into the extracellular space in a process called exocytosis. In this thesis, we developed three new nano- and micro- techniques to study exocytosis. 1. We used scanning ion conductance microscopy (SICM) to image changes in the surface membrane of adrenal chromaffin cells after stimulation of exocytosis. Punctate depressions were noted in clusters of two or more. Increases in membrane surface area, consistent with the fusion and collapse of one or more vesicles into the surface membrane, were observed 64% of the cells. 2. We used a microcontact printing method with PDMS stamps by "soft" lithography to pattern microislands of rat hippocampal neurons to form autapses. Neurons on microstamped microislands survived and grew neurites for more than 21 days and resembled microisland cultures formed by the traditional method of spraying collagen on agarose coated substrates. 3. Microfabricated devices were developed to electrochemically measure quantal catecholamine release from an array of individual cells. Here we report patterning of cellsized holes in [tilde operator] 15 m-thick films. These films are placed on transparent indium tin oxide electrodes to insulate the unused part of the electrode whereas the holes in the film both determine the location of the working electrode and serve as pockets for cell trapping. We found that this approach represents a simple and effective way to target cells to electrodes to record amperometric spikes. en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.subject.lcsh Exocytosis en_US
dc.subject.lcsh Conductometric analysis en_US
dc.subject.lcsh Neurons en_US
dc.subject.lcsh Paraneurons en_US
dc.title Nano- and micro-scale studies of exocytosis en_US
dc.type Thesis en_US
thesis.degree.discipline Biological engineering en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name Ph.D. en_US
thesis.degree.level Doctoral en_US
dc.identifier.merlin .b66671358 en_US
dc.identifier.oclc 316861666 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2007 Dissertations
dc.relation.ispartofcollection 2007 Freely available dissertations (MU)


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