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    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2007 Dissertations (MU)
    • 2007 MU dissertations - Freely available online
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    Identifying the importance of phosphorylation of SNAP-25 at Ser187 in protein kinase C-mediated enhancement of exocytosis

    Shu, Yilong, 1977-
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    [PDF] research.pdf (3.189Mb)
    Date
    2007
    Format
    Thesis
    Metadata
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    Abstract
    Protein Kinase C (PKC) activation has been shown to enhance exocytosis in various studies. However, the molecular mechanism for PKC to promote exocytosis is still elusive. A possible target of PKC is SNAP-25 (25 kDa synaptosome-associated protein), which is a key member of the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) core complex that is essential for exocytosis. Both in vitro and in vivo experiments demonstrate that activation of PKC results in phosphorylation of SNAP-25 at Ser187. However, the importance of SNAP-25 phosphorylation at Ser187 in PKC-mediated enhancement of exocytosis has not been fully studied. Here, I investigated the importance of SNAP-25 phosphorylation at Ser187 upon activation of PKC by a phorbol ester to stimulate exocytosis in rat insulin-secreting INS-1 cells. With the transfection of botulinium toxin E (BoNT/E) to disable the endogenous SNAP-25, my results show that SNAP-25 phosphorylation at Ser187 is important for phorbol ester-enhanced exocytosis. However, my results also indicate that SNAP-25 phosphorylation at Ser187 is not the only mechanism involved in phorbol ester-enhanced exocytosis at high [Ca²+]i. This work helps clarify the molecular mechanisms by which phorbol ester and PKC enhance exocytosis. It also contributes to our understanding of the role of SNAP-25 in exocytosis.
    URI
    https://hdl.handle.net/10355/4757
    https://doi.org/10.32469/10355/4757
    Degree
    Ph. D.
    Thesis Department
    Neuroscience (MU)
    Collections
    • 2007 MU dissertations - Freely available online
    • Interdisciplinary Neuroscience Program electronic theses and dissertations (MU)

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