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dc.contributor.advisorCritser, John Kennetheng
dc.contributor.authorMullen, Steven Francis, 1968-eng
dc.date.issued2007eng
dc.date.submitted2007 Springeng
dc.description"May 2007"eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2007.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Veterinary pathobiology area program.eng
dc.description.abstractHaving effective means to cryopreserve mammalian oocytes could increase the efficiency of managing populations of laboratory animals, increase the effectiveness of breeding programs for livestock, and improve the means by which assisted reproductive therapy is applied to human patients. Unfortunately, for most mammals oocyte cryopreservation suffers from inefficiencies. The work completed in this dissertation was directed at advancing our knowledge of the fundamental cryobiological properties of oocytes from cows, pigs, and humans. The first series of experiments was designed to determine the likelihood of damage to the metaphase II spindle from osmotic stress. Increasing levels of hypotonic and hypertonic stress resulted in an increased proportion of oocytes displaying a damaged spindle as assessed by immunocytochemical staining. Human oocytes appeared more sensitive to hypertonic stress compared to oocytes from cows and pigs. Hypotonic stress caused more damage to cow oocytes compared to human and pig oocytes. Pig oocytes were also shown to lose in vitro developmental potential, and the proportion damaged was greater compared to the proportion showing damage to the spindle. The permeability of mature human oocytes to ethylene glycol and water was also determined. It was shown that the permeability was temperature dependent. The results from the osmotic tolerance and membrane permeability studies for human oocytes was used to develop a theoretically-optimized procedure for vitrifying human oocytes in standard 0.25 cc straws.eng
dc.identifier.merlin.b59291679eng
dc.identifier.oclc163599419eng
dc.identifier.otherMullenS-050107-D6356eng
dc.identifier.urihttp://hdl.handle.net/10355/4804eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2007 Freely available dissertations (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2007 Dissertationseng
dc.subject.lcshCryobiologyeng
dc.subject.lcshOvum -- Cryopreservationeng
dc.subject.lcshMammals -- Reproductioneng
dc.titleAdvances in the fundamental cryobiology of mammalian oocyteseng
dc.typeThesiseng
thesis.degree.disciplineVeterinary pathobiology area program (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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