Advances in the fundamental cryobiology of mammalian oocytes

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Advances in the fundamental cryobiology of mammalian oocytes

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/4804

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dc.contributor.advisor Critser, John Kenneth en
dc.contributor.author Mullen, Steven Francis, 1968- en_US
dc.date.accessioned 2010-01-12T18:42:50Z
dc.date.available 2010-01-12T18:42:50Z
dc.date.issued 2007 en_US
dc.date.submitted 2007 Spring en
dc.identifier.other MullenS-050107-D6356 en_US
dc.identifier.uri http://hdl.handle.net/10355/4804
dc.description "May 2007" en_US
dc.description The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. en_US
dc.description Vita. en_US
dc.description Includes bibliographical references. en_US
dc.description Thesis (Ph. D.) University of Missouri-Columbia 2007. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Veterinary pathobiology area program. en_US
dc.description.abstract Having effective means to cryopreserve mammalian oocytes could increase the efficiency of managing populations of laboratory animals, increase the effectiveness of breeding programs for livestock, and improve the means by which assisted reproductive therapy is applied to human patients. Unfortunately, for most mammals oocyte cryopreservation suffers from inefficiencies. The work completed in this dissertation was directed at advancing our knowledge of the fundamental cryobiological properties of oocytes from cows, pigs, and humans. The first series of experiments was designed to determine the likelihood of damage to the metaphase II spindle from osmotic stress. Increasing levels of hypotonic and hypertonic stress resulted in an increased proportion of oocytes displaying a damaged spindle as assessed by immunocytochemical staining. Human oocytes appeared more sensitive to hypertonic stress compared to oocytes from cows and pigs. Hypotonic stress caused more damage to cow oocytes compared to human and pig oocytes. Pig oocytes were also shown to lose in vitro developmental potential, and the proportion damaged was greater compared to the proportion showing damage to the spindle. The permeability of mature human oocytes to ethylene glycol and water was also determined. It was shown that the permeability was temperature dependent. The results from the osmotic tolerance and membrane permeability studies for human oocytes was used to develop a theoretically-optimized procedure for vitrifying human oocytes in standard 0.25 cc straws. en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.relation.ispartof 2007 Freely available dissertations (MU) en_US
dc.subject.lcsh Cryobiology en_US
dc.subject.lcsh Ovum -- Cryopreservation en_US
dc.subject.lcsh Mammals -- Reproduction en_US
dc.title Advances in the fundamental cryobiology of mammalian oocytes en_US
dc.type Thesis en_US
thesis.degree.discipline Veterinary pathobiology area program en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name Ph. D. en_US
thesis.degree.level Doctoral en_US
dc.identifier.merlin .b59291679 en_US
dc.identifier.oclc 163599419 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2007 Dissertations


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