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dc.contributor.advisorGillis, Kevin D.en
dc.contributor.authorChen, Xiaohui, 1973-en_US
dc.date.issued2007eng
dc.date.submitted2007 Fallen
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from title screen of research.pdf file (viewed on January 30, 2008)en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionVita.en_US
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2007.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.en_US
dc.description.abstractPhotorelease of caged Ca²⁺ is a uniquely powerful tool to study the dynamics of Ca²⁺-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent microchip devices to enable photorelease of caged Ca²⁺ together with electrochemical detection of quantal catecholamine secretion from individual cells or cell arrays as a step towards developing high-throughput experimental devices. A 110 nm - thick transparent Indium-Tin-Oxide (ITO) film was sputter-deposited onto glass coverslips, which were then patterned into 24 cell-sized working electrodes (2̃0 [mu]m by 20 [mu]m). We loaded bovine chromaffin cells with acetoxymethyl (AM) ester derivatives of the Ca²⁺ cage NP-EGTA and Ca²⁺ indicator dye Fura-4F, then transferred these cells onto the working ITO electrodes for amperometric recordings. Upon flash photorelease of caged Ca²+С uniform rise of [Ca²⁺]i within the target cell leads to quantal release of oxidizable catecholamines measured amperometrically by the underlying ITO electrode. We observed a burst of amperometric spikes upon rapid elevation of [Ca²⁺]i and a "priming" effect of sub-stimulatory [Ca²⁺]i on the response of cells to subsequent [Ca²⁺]i elevation, similar to previous reports using different techniques. We conclude that UV photolysis of caged Ca²⁺ is a suitable stimulation technique for higher-throughput studies of Ca²⁺-dependent exocytosis on transparent electrochemical microelectrode arrays.en_US
dc.identifier.merlin.b61978334en_US
dc.identifier.oclc191702789en_US
dc.identifier.otherChenX-092607-D8469en_US
dc.identifier.urihttp://hdl.handle.net/10355/4890
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2007 Freely available dissertations (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2007 Dissertations
dc.subject.lcshExocytosisen_US
dc.subject.lcshChromaffin cellsen_US
dc.subject.lcshCalcium ionsen_US
dc.subject.lcshPhotolithographyen_US
dc.subject.lcshMicrofabricationen_US
dc.subject.lcshGroup 13 elementsen_US
dc.subject.lcshGroup 14 elementsen_US
dc.titleOptical stimulation of quantal exocytosis on transparent microchipsen_US
dc.typeThesisen_US
thesis.degree.disciplineBiological engineeringeng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoralen_US
thesis.degree.namePh. D.en_US


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