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dc.contributor.advisorGillis, Kevin D.eng
dc.contributor.authorChen, Xiaohui, 1973-eng
dc.date.issued2007eng
dc.date.submitted2007 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on January 30, 2008)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionVita.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2007.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.eng
dc.description.abstractPhotorelease of caged Ca²⁺ is a uniquely powerful tool to study the dynamics of Ca²⁺-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent microchip devices to enable photorelease of caged Ca²⁺ together with electrochemical detection of quantal catecholamine secretion from individual cells or cell arrays as a step towards developing high-throughput experimental devices. A 110 nm - thick transparent Indium-Tin-Oxide (ITO) film was sputter-deposited onto glass coverslips, which were then patterned into 24 cell-sized working electrodes (2̃0 [mu]m by 20 [mu]m). We loaded bovine chromaffin cells with acetoxymethyl (AM) ester derivatives of the Ca²⁺ cage NP-EGTA and Ca²⁺ indicator dye Fura-4F, then transferred these cells onto the working ITO electrodes for amperometric recordings. Upon flash photorelease of caged Ca²+С uniform rise of [Ca²⁺]i within the target cell leads to quantal release of oxidizable catecholamines measured amperometrically by the underlying ITO electrode. We observed a burst of amperometric spikes upon rapid elevation of [Ca²⁺]i and a "priming" effect of sub-stimulatory [Ca²⁺]i on the response of cells to subsequent [Ca²⁺]i elevation, similar to previous reports using different techniques. We conclude that UV photolysis of caged Ca²⁺ is a suitable stimulation technique for higher-throughput studies of Ca²⁺-dependent exocytosis on transparent electrochemical microelectrode arrays.eng
dc.identifier.merlin.b61978334eng
dc.identifier.oclc191702789eng
dc.identifier.otherChenX-092607-D8469eng
dc.identifier.urihttp://hdl.handle.net/10355/4890eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshExocytosiseng
dc.subject.lcshChromaffin cellseng
dc.subject.lcshCalcium ionseng
dc.subject.lcshPhotolithographyeng
dc.subject.lcshMicrofabricationeng
dc.subject.lcshGroup 13 elementseng
dc.subject.lcshGroup 14 elementseng
dc.titleOptical stimulation of quantal exocytosis on transparent microchipseng
dc.typeThesiseng
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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