Molecular dissection of meiotic silencing by unpaired DNA
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[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Meiotic silencing by unpaired DNA (MSUD) is a genome surveillance mechanism that uses RNAi to protect against genetic parasites such as retrotransposons and viruses. This is accomplished by scanning the genome for DNA segments not paired with a homologous partner during meiosis and targeting the corresponding messenger RNAs for destruction. MSUD begins when a single-stranded RNA is made from the unpaired region and subsequently converted into small interfering RNA (siRNA), which are used as sequence specific guides for mRNA destruction. To date, nine meiotic silencing genes have been characterized and here we report the identification and characterization of two new candidates that are orthologous to the conserved proteins CBP80 and EPS15.