dc.contributor.advisor | Gates, Kent S. (Kent Stephen), 1962- | eng |
dc.contributor.author | Parsons, Zachary D. | eng |
dc.date.issued | 2015 | eng |
dc.date.submitted | 2015 Spring | eng |
dc.description.abstract | Here, we report on the kinetics and mechanisms associated with redox regulation of the protein tyrosine phosphatase PTP1B. We determined rates of thiol- and phosphinemediated recovery of catalytic activity from the oxidatively-inactivated enzyme, and shed light on the chemical mechanisms involved in these processes and in the regulation of PTP family member SHP2. Additionally, we describe reactions that oxidatively-inactivated PTP1B undergo with structurally-diverse carbon acids, using both a small chemical model dipeptide of oxidatively-inactivated PTP1B, and the oxidized enzyme itself. Finally, we report a novel method by which to predict Hammett substituent constants via electronic structure calculations. | eng |
dc.identifier.uri | https://hdl.handle.net/10355/49109 | |
dc.identifier.uri | https://doi.org/10.32469/10355/49109 | eng |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.rights | OpenAccess. | eng |
dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. | eng |
dc.title | Kinetics and mechanisms associated with redox regulation of protein tyrosine phosphatase 1B (PTP1B) | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Chemistry (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Doctoral | eng |
thesis.degree.name | Ph. D. | eng |