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dc.contributor.advisorSutovsky, Petereng
dc.contributor.authorAntelman, Jennifer L., 1982-eng
dc.date.issued2007eng
dc.date.submitted2007 Springeng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on April 3, 2009)eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2007.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Animal sciences.eng
dc.description.abstractMitochondrial Transcription Factor A (TFAM) is responsible for maintenance and transcriptional control of mitochondrial DNA (mtDNA). TFAM knockout is embryolethal. We have studied the expression and distribution of TFAM in the gametes and preimplantation embryos of a domestic pig (Sus scrofa), a livestock species of major economic significance. We hypothesized that TFAM will be expressed at high levels in oocytes and embryos and that sperm mitochondria should be devoid of TFAM since they are destined for degradation after fertilization. The content of TFAM mRNA increased considerably during porcine oocyte maturation and preimplantation development of porcine embryos. TFAM protein accumulated in the cytoplasm of porcine oocytes during in vitro maturation and was reduced by proteolysis after fertilization. Deviant processing of TFAM protein was observed in parthenogenetically activated oocytes and zygotes reconstructed by somatic cell nuclear transfer (SCNT). Boar sperm TFAM was found to be posttranslationally modified by ubiquitination and relegated to sperm tail principal piece in fully differentiated spermatozoa. These data suggest that mitochondrial transcription factor TFAM is developmentally regulated in porcine gametes and embryos, and may exert a critical role in gametogenesis and preimplantation embryo development. Sperm TFAM protein could be marked for proteolysis by the ubiquitin-proteasome pathway during spermatid elongation to facilitate the degradation of paternal mitochondria after fertilization.eng
dc.identifier.merlinb66700346eng
dc.identifier.oclc317721729eng
dc.identifier.urihttps://hdl.handle.net/10355/5022
dc.identifier.urihttps://doi.org/10.32469/10355/5022eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.subject.lcshMitochondriaeng
dc.subject.lcshGameteseng
dc.subject.lcshOvumeng
dc.subject.lcshSwine -- Embryoseng
dc.titleInvolvement of mitochondrial transcription factor A (TFAM) in porcine gametogenesis and preimplantation embryo developmenteng
dc.typeThesiseng
thesis.degree.disciplineAnimal sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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