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dc.contributor.advisorCone, Karen Camille, 1952-eng
dc.contributor.authorLadipo, Paul B.eng
dc.date.issued2007eng
dc.date.submitted2007 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on September 29, 2008)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.A.) University of Missouri-Columbia 2007.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological sciences.eng
dc.description.abstractCovalent modifications of DNA and nucleosomal histone proteins associated with eukaryotic chromatin have the potential to alter expression of a gene without change in its DNA sequence. One gene regulated through this so-called epigenetic process is the Pl1- Blotched gene of maize. Pl1-Blotched is a allele of the purple1 (pl1) gene, which encodes a transcription factor that activates synthesis of purple anthocyanin pigments. Pl1-Blotched is unusual in that it leads to variegated, rather than uniform, pigmentation. At the molecular level, this phenotype is associated with low expression of pl1 mRNA, a novel pattern of DNA methylation and condensed chromatin structure. To ask if acetylation of nucleosomal histone proteins might be involved in regulating the chromatin organization of Pl1-Blotched, this allele was crossed into 40 lines containing transgenes that target reduction of various histone acetyltransferase and histone deacetylase genes. Some of the lines led to altered Pl1-Blotched pigmentation. Detailed analysis of molecular changes underlying the altered pigmentation resulted in models that implicate both histone acetyltransferase and histone deacetylase genes in controlling expression of Pl1-Blotched. These findings lay the foundation for future studies aimed at further understanding the interplay between histone modification and regulation of gene expression.eng
dc.identifier.merlinb6487882xeng
dc.identifier.oclc259181295eng
dc.identifier.urihttps://hdl.handle.net/10355/5038
dc.identifier.urihttps://doi.org/10.32469/10355/5038eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshHistoneseng
dc.subject.lcshAcetylationeng
dc.subject.lcshDNAeng
dc.subject.lcshGenetic regulationeng
dc.subject.lcshAcetyltransferaseseng
dc.titleThe effects of histone acetylation on the maize allele PL1-blotchedeng
dc.typeThesiseng
thesis.degree.disciplineBiological sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.A.eng


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