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A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
(University of Missouri--Columbia, 2008)
For more than 3 decades, the biochemical details of the Sec system of protein export have been teased out of Escherichia coli. Through these efforts we know that SecA, the ATPase of the Sec system, interacts with several ...
Phenotypic modulation of vascular smooth muscle cells : evidence for a role of the G protein-coupled P2y2 receptor
(University of Missouri--Columbia, 2008)
G protein-coupled receptors regulate two major functions of vascular smooth muscle cells (SMCs) - the vasomotor contractile response and the proliferative response. The contractile phenotype of SMCs has mainly been studied ...
The role of the P2Y₂ nucleotide receptor in inflammation: the mechanisms of P2Y₂ receptor-mediated activation of G proteins
(University of Missouri--Columbia, 2007)
The extracellular ATP/UTP receptor, i.e., the P2Y₂ receptor (P2Y₂R), mediates pro-inflammatory responses in the vasculature, including the endothelium-dependent infiltration of monocytes and their transmigration into sites ...
Regulation of ethylene biosynthesis by mitogen-activated protein kinase cascades in Arabidopsis
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Ethylene (C[subscript2]H[subscript 4]), a simple gaseous hydrocarbon, is an important plant hormone that has profound effects on plant growth, ...
Discovery of epitopes governing MMP-12 specificity for fibrillar substrates : BINDSIght, a method for determining specific sites of substrate surface interactions
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] What determines MMP-12 elastase activity? We have found that the fibrillar substrate specificity of MMP-12 involves surfaces ranging beyond the ...
In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway
(University of Missouri--Columbia, 2009)
Nudt16, previously called X29, is known to be a metal-dependent decapping enzyme expressed in mammals and Xenopus . Nudt16 has been proposed to be the catalytic component of a previously unknown nuclear RNA degradation ...
Characterization of SF-hERR[beta] repression of human estrogen receptor alpha and estrogen receptor-beta transcriptional activities
(University of Missouri--Columbia, 2009)
Discovery of new ways to modulate estrogen actions through estrogen receptor is always exciting because of the importance of estrogen and ERs in many human diseases. In this dissertation, we report our discovery of the ...
Identification of novel breast carcinoma and melanoma avid peptides for imaging
(University of Missouri--Columbia, 2009)
During the last decade, peptides and their derivatives have been employed as effective cancer imaging agents. We aimed to identify new peptides probes specific for melanoma and breast carcinoma by phage display technology. ...
Amyloid beta induces cPLA2 activation by an NADPH oxidase-dependent mechanism in neurons
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Alzheimer's disease (AD) is a neurodegenerative disorder affecting more than 20 million people worldwide. An increase in production of amyloid beta ...
Glomerulosclerosis in the Col1a2-deficient mouse model : homotrimer pathogenesis and MMP expression
(University of Missouri--Columbia, 2009)
The Col1a2-deficient (oim) mouse model exclusively synthesizes homotrimeric type I collagen due to the lack of functional pro [alpha] 2(I) collagen chains. The mouse develops a type I collagen glomerulopathy that has ...
A custom oligonucleotide microarray analysis as a tool for dissecting soybean-bradyrhizobium japonicum nodule senescence
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Soybean root nodules are symbiotic organs housing Bradyrhizobium japonicum, a soil bacterium that reduces atmospheric nitrogen to ammonium. The bacterium ...
Regulation of Nrf2 by a keap1-dependent E3 ubiquitin ligase
(University of Missouri--Columbia, 2007)
Oxidative damage to biological macromolecules has been implicated in diverse pathophysiological processes, including cancer, neurodegeneration, aging and cardiovascular disease. The Nrf2 transcription factor regulates ...
Understanding Arabidopsis ion homeostasis in the post-genomic era : assigning function to two proteins involved in iron metabolism
(University of Missouri--Columbia, 2006)
Two projects studying different aspects of iron deficiency in the model plant Arabidopsis thaliana are detailed here. The first project describes the isolation and characterization of the Arabidopsis frd4-1 and frd4-2 ...
Glomerular deposition of homotrimeric type I collagen in the COL1A2 deficient mouse
(University of Missouri--Columbia, 2006)
Type I collagen is the most abundant structural protein in the body. Type I collagen generally exists as a heterotrimeric protein; however, a homotrimeric isotype of type I collagen has been identified. Our lab currently ...
The use of FD-filamentous bacteriophage for the in vivo imaging of cancer
(University of Missouri--Columbia, 2006)
Improved diagnosis of cancer through targeted imaging of neoplasms is needed for early detection and treatment of cancer. We propose that the physical characteristics of filamentous bacteriophage (phage) are well suited ...
The role of P2Y₂ nucleotide receptors in vascular inflammation
(University of Missouri--Columbia, 2007)
Extracellular nucleotides act on P2 receptors to regulate vascular tone. Large amounts of extracellular nucleotides are released at the sites of tissue injury and may play an important role in the vascular inflammation. ...
Protein phosphorylation regulation in Arabidopsis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Protein phosphorylation and dephosphorylation are fundamental mechanisms involved in many cell-signaling events. Coordinated actions of protein kinases ...
Crystal structure of the kelch domain of human keap1
(University of Missouri--Columbia, 2005)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Nrf2-Keap1 signal transduction pathway senses oxidative stress and protects eukaryotic cells against oxidative damage and cancer. The transcription ...
Physiologic and metabolic interactions in the soybean/bradyrhizobium japonicum symbiosis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The agronomically vital, nitrogen-fixing symbioses between soybean and the soil bacterium Bradyrhizobium japonicum is a model system for the study of ...
GABP regulation of the murine GABPa/ATPsynthase coupling factor six and human glutathione reductase promoters
(University of Missouri--Columbia, 2005)
GABPa is expressed from a bi-directional promoter expressing ATP synthase coupling factor six (CF6) in the opposite direction. This bi-directional promoter is regulated by GABP, indicating an autoregulation of GABPa. GABP ...