Biochemistry electronic theses and dissertations (MU): Recent submissions
Now showing items 21-40 of 104
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The musculoskeletal effect of exercise and soluble activin receptor type 2b in mouse models of osteogenesis imperfecta
(University of Missouri--Columbia, 2017)[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Osteogenesis Imperfecta (OI) is a heritable connective tissue disorder primarily due to mutations in the type I collagen containing tissues, such as ... -
Advancing single molecule fluorescence microscopy through the study of dynamic macromolecules
(University of Missouri--Columbia, 2017)We took a two pronged approach to our investigation of protein synthesis by the ribosome: 1) the generation of a novel fluorescence enhancing substrates 2) the development of a method for the generation of fluorescent ... -
The role of the P2X7 nucleotide receptor in salivary gland inflammation
(University of Missouri--Columbia, 2017)Salivary gland inflammation is a hallmark of Sjogren's syndrome (SS), a common autoimmune disease characterized by lymphocytic infiltration and the consequent impairment of the salivary gland and loss of saliva secretion, ... -
The influence of mRNA secondary structures on ribosome conformational dynamics
(University of Missouri--Columbia, 2016)[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Synthesizing proteins in the cell is a critical aspect of life. Protein synthesis is a complicated process and involve highly functional machines at a ... -
Kinetic study of histidine kinase CheA in bacterial chemotaxis
(University of Missouri--Columbia, 2017)Histidine kinase CheA is central to signaling in bacterial chemotaxis. This kinase is responsible for the phosphorylation of two response regulators, CheY and CheB. CheY controls flagellar rotation and thus motility. CheB ... -
Discovery and characterization of regulatory mechanisms affecting the heteromeric acetyl-coenzyme a carboxylase in Arabidopsis
(University of Missouri--Columbia, 2016)Fatty acid biosynthesis (FAS) is an essential metabolic pathway used by all organisms to generate fatty acids. A staple component of this pathway is the enzyme acetyl-CoA carboxylase (ACCase), which catalyzes the committed ... -
Ribozymes and aptamers in the RNA world, and in synthetic biology
(University of Missouri--Columbia, 2016)[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The RNA world hypothesis postulates that Ribonucleic Acids (RNA) may have provided functions of catalysis and genetic information storage during the ... -
Advances in aptamer evolution and engineering
(University of Missouri--Columbia, 2016)[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Aptamers are single-stranded nucleic acids that fold into unique three-dimensional shapes that allow them to bind with high affinity and specificity ... -
Interaction of secretory protein SecA with its binding partners : lipids and SecB
(University of Missouri--Columbia, 2016)Thirty percent of proteins synthesized in the cytoplasm of E.coli are exported across or into the cytoplasmic membrane to reach their final destinations. The general secretory system exports precursor proteins through the ... -
Study of PVCA and PVCB, two enzymes involved in making isonitrile-containing natural products in bacterial pathogens
(University of Missouri--Columbia, 2016)Isonitriles, with an unusual functionality, are very versatile intermediates widely involved in organic synthesis. Interestingly, a variety of isonitriles were encountered in both marine and terrestrial systems as the ... -
Stability, states, and interactions of the enzyme PMM/PGM by NMR and multivariate analysis
(University of Missouri--Columbia, 2016)Phosphomannomutase/phosphoglucomutase (PMM/PGM) contributes to the infectivity of an opportunistic human pathogen Pseudomonas aeruginosa by participating in carbohydrate biosynthesis. As a phospho-transfer enzyme, PMM/PGM ... -
Protein components of the microbial mercury methylation pathway
(University of Missouri--Columbia, 2015)Mercury (Hg) in the environment and the resulting methylmercury (CH3Hg+) produced by microorganisms has emerged as a global concern in the last 50 years after a series of high profile, large scale contamination events, ... -
Structural studies of inhibition and resistance mechanism of EFDA, a human immunodeficiency virus reverse transcriptase inhibitor
(University of Missouri--Columbia, 2016)[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] -
Characterization of 14-3-3 [chi] phosphorylation in phosphorus stress in Arabidopsis thaliana and investigation of acetyl-coA carboxylase regulation using quantitative mass spectrometry
(University of Missouri--Columbia, 2016)Oilseed development involves coordination and regulation of a complex metabolic network for efficient production of storage compounds, including fatty acids, proteins, and carbohydrates. Recent advances in mass spectrometry ... -
Role of the P2Y2 nucleotide receptor in inflammation and inflammatory diseases
(University of Missouri--Columbia, 2015)Nucleotides are released from cells in response to tissue damage and activate P2X and P2Y receptors on the cell surface to regulate inflammation, a critical process for wound repair. During inflammation, leukocytes are ... -
Transient collagen triple-helix binding to membrane type 1 matrix metalloproteinase : interaction studies and NMR-guided structural docking
(University of Missouri--Columbia, 2015)[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] MT1-MMP (MMP-14) as pericellular collagenase is critically involved in cancer cell invasion through collagen barriers that it degrades. To better ... -
Characterizing the retroviral envelope glycoprotein membrane proximal external region and membrane-spanning domains for their roles in helical alignment, fusogenicity, and incorporation into viral particles.
(University of Missouri--Columbia, 2015)Viruses use surface proteins to gain access into target cells to initiate infection. How these surface proteins make it into assembling viral particles and the exact details of the entry process are poorly understood. My ... -
Characterization of Esrrb function in metastatic prostate cancer cells and transcriptional regulation of hedgehog-signaling pathway target genes
(University of Missouri--Columbia, 2014)Orphan nuclear receptor Estrogen Receptor Related Receptor Beta (Esrrb) is a transcription factor. Although it was also shown to be important in cancer, little is known about its function in cancer cells and cancer relevant ... -
Nitrate and nitrite growth inhibition of Desulfovibrio strains
(University of Missouri--Columbia, 2014)Sulfate-reducing bacteria, common non-pathogenic soil bacteria, have the ability to limit the movement of toxic heavy metals from one environmental site to another by keeping those metals from dissolving in water. Limiting ... -
Role of copper in defending against bacterial infection
(University of Missouri--Columbia, 2015)[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Copper is an essential nutrient. It plays an important role in development, pigmentation, neurological function, and immune defense. Copper deficiency ...