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High-throughput genomics and the Sword of Damocles
(2010-03)
Next-generation sequencing and high-throughput assaying technologies have dichotomized entire research communities into those individuals who have rapidly evolved in response to the technological selection pressure and ...
Improvement of feed efficiency in beef cattle through selection upon residual feed intake (RFI)
(University of Missouri--Columbia, 2010)
Selecting beef cattle which metabolically are more efficient at converting feed into gain or body weight maintenance is one way to reduce feed cost in beef production. Residual feed intake (RFI) is one trait used to identify ...
Swab Test Using ELISA Technique for Diagnosis of Brown Recluse Bites
(2010-03)
Envenomations by the brown recluse spider, Loxosceles reclusa, are a significant source of morbidity and occasional mortality in endemic regions of the United States, and misdiagnoses are common. A survey of physicians ...
Cloning & Transgenic Swine for Medical and Agricultural Uses at MU
(2010-03)
The pig is an important component of the world's food supply. As of December 1, 2009 the hog inventory in the United States of America was 65 million head. The United States is the world's third largest producer and second ...
Differential gene and protein expression in pig nuclear transfer extraembryonic membranes and blastocyst stage embryos
(University of Missouri--Columbia, 2010)
Nuclear transfer (cloning) is the process of transferring a donor cell nucleus into an enucleated oocyte. The oocyte and donor cell are subsequently fused and activated to allow for nuclear reprogramming and continued ...
Alleviation of heat stress with Tasco in dairy cows
(University of Missouri--Columbia, 2010)
The present study determined the impact of Tasco, Ascophyllum nodosum, on heat stress in dairy cows. Thirty-two Holstein cows were utilized in a randomized complete block design, during 63 days in summer 2008. Feed intake, ...
Determination of fat percentage using three different methods within marbling scores on beef longissimus muscle
(University of Missouri--Columbia, 2010)
Consumers are concerned with fat consumption from meat products and the ability to determine fat has changed with recent technological advances. The objective of this study was to determine fat percentage within marbling ...
Derivation and characterization of LIF and FGF2 dependent Porcine Induced Pluripotent Stem Cells [abstract]
(2010-02)
Authentic embryonic stem cells (ESC) have probably never been derived from the inner cell mass (ICM) of pig, despite over 25 years of effort. Recently, several groups, including ours, have reported derivation of induced ...
Commercialization of novel biomarkers of male fertility in humans and farm animals
(2010-03)
Each year, US infertility clinics treat 135,000 couples who fail to conceive naturally. Up to 40% of these infertility cases can be attributed to male infertility stemming from poor sperm quality. An additional 20% of ...
Swine models of Human Conditions
(2010-03)
Since the introduction of Dolly, the first cloned sheep, genetic engineering of livestock has advanced to the point that nearly any genetic modification appears feasible. Genes can be introduced, deleted or altered in ...
Effects of Artemisia afra and Artemisia absinthium on fertility of male mice exposed to increased ambient temperatures for 24 hours
(University of Missouri--Columbia, 2010)
Males exposed to elevated temperatures display a decrease in reproductive performance resulting in decreased profit for producers. The use of herbs to alleviate the effects of heat in tropical countries has been documented ...
Porcine induced pluripotent stem cells (piPSC) for expanding the use of swine in biomedical research
(2010-03)
Our goal is to create porcine pluripotent stem cells, i.e. ones capable of differentiating into all cell types of the body that can expand the use of swine as a biomedical model for studying human disease. It is well ...
Ruminal degradation of protein and carbohydrate in the domestic and wild ruminant
(University of Missouri--Columbia, 2010)
To optimize growth and health ruminant animals in a confined setting, diets need to be formulated to provide balanced levels of rumen degradable energy and nitrogen (RDN) to maximize microbial growth while also providing ...
Characterization of progesterone receptor (PGR) mRNA and protein isoforms in the endometrium of cyclic and pregnant pigs
(University of Missouri--Columbia, 2010)
Disappearance of the progesterone receptor (PGR) from the uterine luminal epithelium (LE) is essential for cyclicity and pregnancy in pigs. In humans, three PGR mRNA isoforms (PGR-A, PGR-B and PGR-C) arise from alternative ...
Effect of ovulatory follicle size on bovine pregnancy associated glycoproteins in beef cattle
(University of Missouri--Columbia, 2010)
The GnRH-induced ovulation of small dominant follicles was associated with reduced pregnancy rates and late embryonic/fetal survival around the time of embryo-uterine attachment. PAGs are secreted by binucleated trophoblast ...
The role of cytoplasmic polyadenylation element sequence on mRNA abundance in porcine embryogenesis
(University of Missouri--Columbia, 2010)
Development of a porcine germinal vesicle oocyte (GVO) to a 4-cell stage embryo occurs during a transcriptionally silent period when the oocyte/embryo relies on maternally derived mRNA to encode proteins required for ...
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
(University of Missouri--Columbia, 2010)
In vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos ...
Efficacy of interferon-stimulated genes and pregnancy-associated glycoproteins for pregnancy diagnosis within an estrus resynchronization protocol for dairy cattle
(University of Missouri--Columbia, 2010)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Reproductive management is a major economic concern in the dairy industry. Long intervals between TAI increases the days open and decrease the 21 day ...
Approaches for alleviating toxic effects of aflatoxin in lactating dairy cows and weanling pigs
(University of Missouri--Columbia, 2010)
Aflatoxin (AF) is a mycotoxin found in feed ingredients used in livestock diets including dairy cattle and pigs. Aflatoxin causes reduced performance, liver damage, increased prevalence of infectious disease, and secretion ...
Heritable, differential gene expression in pre-implantation embryos from rats with endometriosis and their daughters
(University of Missouri--Columbia, 2010)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Endometriosis, which affects millions of women, is a condition where the uterine lining grows outside of the uterus and is associated with subfertility ...