Chromatin-level regulation of the maize purple plant1 gene

MOspace/Manakin Repository

Breadcrumbs Navigation

Chromatin-level regulation of the maize purple plant1 gene

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/5577

[+] show full item record


Title: Chromatin-level regulation of the maize purple plant1 gene
Author: Shin, KyungJu.
Date: 2008
Publisher: University of Missouri--Columbia
Abstract: In eukaryotes, gene expression is controlled at the chromatin level by mechanisms that are not yet well understood. This study addressed some of the unanswered questions about chromatin-level gene regulation. In maize, the Pl1- Blotched allele of purple plant1 (pl1) leads to a variegated pattern of anthocyanin pigmentation. Pigmentation of Pl1-Blotched plants is increased by presence of the modifier Suppressor of plant blotching (Spb), and the higher pigment level is correlated with increased Pl1-Blotched expression and altered DNA methylation. These observations led to the hypothesis that Spb modulates Pl1-Blotched expression by altering the chromatin structure of the gene. To evaluate this idea, DNase I sensitivity and chromatin immunoprecipitation assays were used to analyze the effect of Spb on Pl1-Blotched chromatin structure and QTL mapping was used to estimate the number of genes Spb comprises. Results suggest that Spb activates Pl1-Blotched expression by chromatin remodeling, which leads to more relaxed chromatin structure, elevated levels of "active" histone modifications, and altered nucleosome positioning. Moreover, the data suggest that these effects are brought about by multiple proteins that act together to modify Pl1-Blotched chromatin. The insights provided by this study have led to increased understanding of chromatin-level regulation of gene expression.
URI: http://hdl.handle.net/10355/5577
Other Identifiers: ShinK-051208-D9881

This item appears in the following Collection(s)

[+] show full item record