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    • Graduate School - MU Theses and Dissertations (MU)
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    • 2008 Dissertations (MU)
    • 2008 MU dissertations - Freely available online
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    Chromatin-level regulation of the maize purple plant1 gene

    Shin, KyungJu.
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    Date
    2008
    Format
    Thesis
    Metadata
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    Abstract
    In eukaryotes, gene expression is controlled at the chromatin level by mechanisms that are not yet well understood. This study addressed some of the unanswered questions about chromatin-level gene regulation. In maize, the Pl1- Blotched allele of purple plant1 (pl1) leads to a variegated pattern of anthocyanin pigmentation. Pigmentation of Pl1-Blotched plants is increased by presence of the modifier Suppressor of plant blotching (Spb), and the higher pigment level is correlated with increased Pl1-Blotched expression and altered DNA methylation. These observations led to the hypothesis that Spb modulates Pl1-Blotched expression by altering the chromatin structure of the gene. To evaluate this idea, DNase I sensitivity and chromatin immunoprecipitation assays were used to analyze the effect of Spb on Pl1-Blotched chromatin structure and QTL mapping was used to estimate the number of genes Spb comprises. Results suggest that Spb activates Pl1-Blotched expression by chromatin remodeling, which leads to more relaxed chromatin structure, elevated levels of "active" histone modifications, and altered nucleosome positioning. Moreover, the data suggest that these effects are brought about by multiple proteins that act together to modify Pl1-Blotched chromatin. The insights provided by this study have led to increased understanding of chromatin-level regulation of gene expression.
    URI
    https://doi.org/10.32469/10355/5577
    https://hdl.handle.net/10355/5577
    Degree
    Ph. D.
    Thesis Department
    Biological sciences (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • 2008 MU dissertations - Freely available online
    • Biological Sciences electronic theses and disserations (MU)

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