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dc.contributor.advisorReddy, Chada S.eng
dc.contributor.advisorKatti, Kattesh V.eng
dc.contributor.authorMekapothula, Swapnaeng
dc.date.issued2008eng
dc.date.submitted2008 Springeng
dc.description"May 2008"eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2008.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Veterinary biomedical sciences.eng
dc.description.abstractThis research focuses on conjugation of two major biomolecules i.e., Bombesin and Annexin V, to AuNps and studying their target specificity. The target specificity of AuNps coated with Bombesin, a GRP receptor specific protein was tested using two cancer cell lines (MCF-7and PC-3) that over-express GRP receptors. Both cell types exhibit significant uptake of Bombesin gold nanoparticles, internalizing them through a highly specific receptor mediated endocytosis pathway. Binding of AuNps coated with a phospholipid-binding protein Annexin V with high affinity towards apoptotic cells was tested in Jurkat-T- lymphocytes. Annexin VA[Mu]Nps showed excellent affinity towards the apoptotic Jurkat-T lymphocytes binding to the cells in a manner similar to the biomolecule annexin V. We further used environmentally benign so called green chemicals i.e., present in tea, soybean, and cinnamon and their synergistic reduction potentials to reduce the gold salts into AuNps. Such nanoparticles also showed excellent affinity toward epidermal growth factor receptors (EGFR) on prostate and breast cancer cells and proved to be noncytotoxic at as high as 150[Mu]M. These studies showed that gold nanoparticles can be coated not only to exert specific molecular interactions in specific cell types but also to be devoid of adverse effects.eng
dc.identifier.merlinb6705870xeng
dc.identifier.oclc319171126eng
dc.identifier.urihttps://hdl.handle.net/10355/5641
dc.identifier.urihttps://doi.org/10.32469/10355/5641eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2008 Freely available theses (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2008 Theseseng
dc.subject.lcshNanoparticleseng
dc.subject.lcshGold -- Molecular aspectseng
dc.subject.lcshBiomedical materialseng
dc.subject.lcshBiocompatibilityeng
dc.subject.lcshBombesineng
dc.subject.lcshLipocortinseng
dc.subject.lcshCancer cells -- Effect of drugs oneng
dc.titleGold nanoparticle-biomolecule conjugates: synthesis, properties, cellular interactions and cytotoxicity studieseng
dc.typeThesiseng
thesis.degree.disciplineVeterinary biomedical sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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