[-] Show simple item record

dc.contributor.advisorIslam, Naz E.eng
dc.contributor.authorDonthula, Vinithaeng
dc.date.issued2008eng
dc.date.submitted2008 Falleng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on September 22, 2009).eng
dc.descriptionThesis advisor: Dr. Naz E. Islam.eng
dc.descriptionM.S University of Missouri--Columbia 2008.eng
dc.description.abstractThe effects of Pulsed Electric Fields have been a period of study since past few decades, which was mainly on the electroporation, bacterial decontamination and biofouling. But the development of technology to produce nanosecond duration pulsed electric fields has allowed analysis of the effects of ultrashort duration, high intensity electric fields on living cells. In this thesis we investigated the effects of nanosecond pulsed electric fields (nsPEF) on the protein concentration, DNA concentration and 11 [beta] Hydroxysteroid Dehydrogenase type 2 (11[beta]HSD2) enzyme activity in human prostate cancer cell line - LNCaP nsPEF application of pulse duration 60 ns with electric field intensity of 32.5kV/cm were applied to cells in train of 1, 5, or 10 pulses. The protein concentration was determined by a Bio-Rad DC protein assay, while Hoechst 33258 was useful in quantitative determination of DNA in these cells. Both the protein concentration and DNA concentration declined by 18.77% (1 Pulse) and 19.04 % (10 Pulse) compared to Controls. LNCaP cells showed a decrease in DNA of 5.29 % (1 Pulse), 8.95 % (5 Pulse) and 18.36 % (10 Pulses) compared to Controls. The enzyme 11[beta]HSD2, which is present in the LNCaP cell culture also showed a reduction in activity supporting our hypothesis that the ultrashort pulses are affecting the intracellular structures.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.identifier.merlinb71028912eng
dc.identifier.oclc437417031eng
dc.identifier.urihttps://doi.org/10.32469/10355/5665eng
dc.identifier.urihttps://hdl.handle.net/10355/5665
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2008 Theseseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subject.lcshElectroporationeng
dc.subject.lcshProstate -- Cancereng
dc.subject.lcshCancer cells -- Growth -- Regulationeng
dc.subject.lcshNanomedicineeng
dc.subject.lcshSurfaces (Technology)eng
dc.titleEffects of nano-second pulsed electric fields (nsPEF) on human prostate cancer cell line - LNCaPeng
dc.typeThesiseng
thesis.degree.disciplineElectrical engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record