Microbial pathogen contamination in mouse gametes and embryos

MOspace/Manakin Repository

Breadcrumbs Navigation

Microbial pathogen contamination in mouse gametes and embryos

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/5671

[-] show simple item record

dc.contributor.advisor Riley, Lela K. (Lela Kay) en_US
dc.contributor.author Zhang, Lin, 1974 Dec. 4- en_US
dc.date.accessioned 2010-02-23T17:13:49Z
dc.date.available 2010-02-23T17:13:49Z
dc.date.issued 2008 en_US
dc.date.submitted 2008 Spring en
dc.identifier.other ZhangL-050508-T10121 en_US
dc.identifier.uri http://hdl.handle.net/10355/5671
dc.description Includes bibliographical references. en_US
dc.description Thesis (M.S.) University of Missouri-Columbia 2008. en_US
dc.description The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. en_US
dc.description "May 2008" en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- microbiology (Medicine). en_US
dc.description.abstract Large numbers of transgenic and knockout mice have been generated. Cryopreservation provides an efficient and effective way to maintain these unique genetically modified rodent lines. However, there is a great risk of transmitting various diseases via the cryopreserved gametes and embryos. To investigate which of the commonly found murine pathogens contaminate mouse gametes and embryos, seven commonly found pathogens in research mice were evaluated in semen, oocyte-cumulus complexes and embryos collected from naturally infected mice in the first part of my study. These seven pathogens are: mouse parvovirus, Helicobacter, Mycoplasma pulmonis, Pasteurella pneumotropica, murine norovirus, mouse hepatitis virus and Theiler's murine encephalomyelitis virus. Five pathogens, mouse parvovirus, Helicobacter, mouse hepatitis virus, murine norovirus, Theiler's encephalomyelitis virus, were found in mouse gametes and embryos. These findings indicate there is a potential risk of transmitting microbial pathogens via cryopreserved gametes and embryos. The second part of this study focused on evaluating mouse parvovirus and determining the effect of infection in male mice on in vitro fertilization including how it will change fertilization rate, cleavage rate and embryonic development and whether mouse parvovirus transmits to embryos at blastocyst stage. We did not find mouse parvovirus-1 infection in male mice transmitted to in vitro produced embryos and there was no effect on fertilization rate, cleavage rate and embryonic development following in vitro fertilization. en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.relation.ispartof 2008 Freely available theses (MU) en_US
dc.subject.mesh Mice -- microbiology en_US
dc.subject.mesh Ovum -- microbiology en_US
dc.subject.mesh Embryo, Mammalian -- microbiology en_US
dc.subject.mesh Rodent Diseases -- epidemiology en_US
dc.subject.mesh Spermatozoa -- microbiology en_US
dc.subject.mesh Cryopreservation en_US
dc.title Microbial pathogen contamination in mouse gametes and embryos en_US
dc.type Thesis en_US
thesis.degree.discipline Microbiology (Medicine) en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name M.S. en_US
thesis.degree.level Masters en_US
dc.identifier.merlin .b64866944 en_US
dc.identifier.oclc 256500425 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2008 Theses


This item appears in the following Collection(s)

[-] show simple item record