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dc.contributor.advisorGangopadhyay, Shubhraeng
dc.contributor.authorSen, Atanueng
dc.date.issued2008eng
dc.date.submitted2008 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on August 14, 2009)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2008.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.eng
dc.description.abstractA high throughput lab-on-a-chip device is being developed for single cell capture for the purpose of high time resolution quantal exocytosis measurement with high probability of cell docking. This device makes use of DLC (Diamond like Carbon) deposited on a conducting ITO (Indium Tin Oxide) film to bring about a suitable electrode incorporating biocompatibility, transparency and low resistivity and electrochemical activity to enhance cell docking and detect catecholamine release. Various materials have been tested for their ability to promote cell attachment. Diamond like Carbon being an established biocompatible and cytophilic material has been compared with other metal electrodes for cell attachment using an assay developed for this study. Cells tested for attachment were either the insulin-secreting cell line INS-1 or catecholamine-secreting bovine chromaffin cells. With either cell type, I found that the rank order of cell attachment following overnight culture was DLC [greater than] ITO, Pt [greater than] Au. Teflon has also tested as a candidate insulating material to prevent cell attachment outside of this docking site. The cell attachment can be enhanced by coating of poly-d-lysine on the metal while retaining the electrochemical activity of the metal electrode. The fraction of cells that were dead following overnight culture were similar among the tested material. In summary, my results suggest that electrodes fabricated from poly-d-lysine coated DLC insulated with Teflon will selectively promote attachment of cells to measurement electrodes.eng
dc.identifier.merlin.b70640385eng
dc.identifier.oclc430536454eng
dc.identifier.otherSenA-032509-T11543eng
dc.identifier.urihttp://hdl.handle.net/10355/5722eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshExocytosiseng
dc.subject.lcshBioMEMSeng
dc.subject.lcshCatecholamineseng
dc.titleQuantification of cell attachment on different materials as candidate electrodes for measurement of quantal exocytosiseng
dc.typeThesiseng
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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