[-] Show simple item record

dc.contributor.advisorGangopadhyay, Shubhraen
dc.contributor.authorSen, Atanuen_US
dc.date.issued2008eng
dc.date.submitted2008 Summeren
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from title screen of research.pdf file (viewed on August 14, 2009)en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2008.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.en_US
dc.description.abstractA high throughput lab-on-a-chip device is being developed for single cell capture for the purpose of high time resolution quantal exocytosis measurement with high probability of cell docking. This device makes use of DLC (Diamond like Carbon) deposited on a conducting ITO (Indium Tin Oxide) film to bring about a suitable electrode incorporating biocompatibility, transparency and low resistivity and electrochemical activity to enhance cell docking and detect catecholamine release. Various materials have been tested for their ability to promote cell attachment. Diamond like Carbon being an established biocompatible and cytophilic material has been compared with other metal electrodes for cell attachment using an assay developed for this study. Cells tested for attachment were either the insulin-secreting cell line INS-1 or catecholamine-secreting bovine chromaffin cells. With either cell type, I found that the rank order of cell attachment following overnight culture was DLC [greater than] ITO, Pt [greater than] Au. Teflon has also tested as a candidate insulating material to prevent cell attachment outside of this docking site. The cell attachment can be enhanced by coating of poly-d-lysine on the metal while retaining the electrochemical activity of the metal electrode. The fraction of cells that were dead following overnight culture were similar among the tested material. In summary, my results suggest that electrodes fabricated from poly-d-lysine coated DLC insulated with Teflon will selectively promote attachment of cells to measurement electrodes.en_US
dc.identifier.merlin.b70640385en_US
dc.identifier.oclc430536454en_US
dc.identifier.otherSenA-032509-T11543en_US
dc.identifier.urihttp://hdl.handle.net/10355/5722
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2008 Freely available theses (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2008 Theses
dc.subject.lcshExocytosisen_US
dc.subject.lcshBioMEMSen_US
dc.subject.lcshCatecholaminesen_US
dc.titleQuantification of cell attachment on different materials as candidate electrodes for measurement of quantal exocytosisen_US
dc.typeThesisen_US
thesis.degree.disciplineBiological engineeringeng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMastersen_US
thesis.degree.nameM.S.en_US


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record