Quantification of cell attachment on different materials as candidate electrodes for measurement of quantal exocytosis

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Quantification of cell attachment on different materials as candidate electrodes for measurement of quantal exocytosis

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/5722

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dc.contributor.advisor Gangopadhyay, Shubhra en
dc.contributor.author Sen, Atanu en_US
dc.date.accessioned 2010-02-23T17:14:54Z
dc.date.available 2010-02-23T17:14:54Z
dc.date.issued 2008 en_US
dc.date.submitted 2008 Summer en
dc.identifier.other SenA-032509-T11543 en_US
dc.identifier.uri http://hdl.handle.net/10355/5722
dc.description The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. en_US
dc.description Title from title screen of research.pdf file (viewed on August 14, 2009) en_US
dc.description Includes bibliographical references. en_US
dc.description Thesis (M.S.) University of Missouri-Columbia 2008. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Biological engineering. en_US
dc.description.abstract A high throughput lab-on-a-chip device is being developed for single cell capture for the purpose of high time resolution quantal exocytosis measurement with high probability of cell docking. This device makes use of DLC (Diamond like Carbon) deposited on a conducting ITO (Indium Tin Oxide) film to bring about a suitable electrode incorporating biocompatibility, transparency and low resistivity and electrochemical activity to enhance cell docking and detect catecholamine release. Various materials have been tested for their ability to promote cell attachment. Diamond like Carbon being an established biocompatible and cytophilic material has been compared with other metal electrodes for cell attachment using an assay developed for this study. Cells tested for attachment were either the insulin-secreting cell line INS-1 or catecholamine-secreting bovine chromaffin cells. With either cell type, I found that the rank order of cell attachment following overnight culture was DLC [greater than] ITO, Pt [greater than] Au. Teflon has also tested as a candidate insulating material to prevent cell attachment outside of this docking site. The cell attachment can be enhanced by coating of poly-d-lysine on the metal while retaining the electrochemical activity of the metal electrode. The fraction of cells that were dead following overnight culture were similar among the tested material. In summary, my results suggest that electrodes fabricated from poly-d-lysine coated DLC insulated with Teflon will selectively promote attachment of cells to measurement electrodes. en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.subject.lcsh Exocytosis en_US
dc.subject.lcsh BioMEMS en_US
dc.subject.lcsh Catecholamines en_US
dc.title Quantification of cell attachment on different materials as candidate electrodes for measurement of quantal exocytosis en_US
dc.type Thesis en_US
thesis.degree.discipline Biological engineering en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name M.S. en_US
thesis.degree.level Masters en_US
dc.identifier.merlin .b70640385 en_US
dc.identifier.oclc 430536454 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2008 Theses
dc.relation.ispartofcollection 2008 Freely available theses (MU)


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