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dc.contributor.advisorHassett, Daniel E.eng
dc.contributor.authorThoma, Michelle C.eng
dc.date.issued2008eng
dc.date.submitted2008 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.description"August 2008"eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2008.eng
dc.description.abstractDue to their ease of production and safety, plasmid-based vaccines have become prime candidates for both prophylactic and therapeutic human vaccination. In experimental animal models, vaccination with plasmid DNA expression vectors has been shown to induce robust cellular and humoral immune responses against a variety of infectious diseases and some cancers. However, DNA vaccines have proven to be weakly immunogenic in human clinical trials. The poor immunogenicity of DNA vaccines in humans necessitates the development of novel methods to enhance both cellular and humoral immune responses against the plasmid-encoded antigen(s). Previous in vivo studies have shown that CD4+CD25+Foxp3+ T regulatory cells can repress antigenspecific immune responses. We demonstrate here that depletion of CD25+ cells prior to plasmid vaccination significantly enhances primary and memory T cell responses and antibody responses to a model DNA vaccine against Lymphocytic choriomeningitis virus. If this approach can be safely applied to humans it may not only improve the clinical utility of DNA vaccines but also improve conventional vaccines as well.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.identifier.merlinb66799715eng
dc.identifier.oclc318898565eng
dc.identifier.urihttps://doi.org/10.32469/10355/5764eng
dc.identifier.urihttps://hdl.handle.net/10355/5764
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2008 Theseseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subject.lcshPlasmids -- Immunologyeng
dc.subject.lcshDNA vaccineseng
dc.subject.lcshInterleukin-2 -- Receptorseng
dc.subject.lcshLymphocytic choriomeningitiseng
dc.subject.lcshT cellseng
dc.subject.meshPlasmids -- immunologyeng
dc.subject.meshVaccines, DNA -- immunologyeng
dc.subject.meshInterleukin-2 Receptor alpha Subunit -- immunologyeng
dc.subject.meshLymphocytic Choriomeningitis -- immunologyeng
dc.subject.meshT-Lymphocytes -- immunologyeng
dc.titleRegulating the regulators : using CD25 depletion to enhance immune responses to a model plasmid-based vaccineeng
dc.typeThesiseng
thesis.degree.disciplineMicrobiology (Medicine) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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