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    Aptamer encoded nanopores as single molecule sensors

    Ding, Shu
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    [PDF] research.pdf (2.039Mb)
    Date
    2008
    Format
    Thesis
    Metadata
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    Abstract
    We have created a low noise, calibrated, molecular-scaled pore. The nanopore is formed either by micro-forge polishing or external penetration of the nanocavity sealed in the pipette terminal. The nanopore fabrication is both cost-effective and time-efficient. The uniform profile of the nanocavity is imaged by a scanning electron microscope (SEM). It suggested a molecular scale pore size and conical pore shape. By correlating pore size and conductance, we have established a method for calibrating pore size by conductance level. The pore diameter was further verified by the translocation of double-strand DNA (dsDNA), which has a known size 2̃nm. Our study shows that glass nanopore can be modified with DNA/RNA aptamer as a promising biosensor. Single molecules (IgE, Ricin and streptavidin/biotin) were detected rapidly and sensitively in real time electrical measurement. Simultaneous recording showed a dynamic process in molecule recognition and interaction. This demonstrated that a glass nanopore could impact single protein molecule detection for medical and biothreat applications. One of the exciting observations is a single restriction enzyme (Hind III) activity within nano-confinement. The duration of event could indicate the strength of the interaction and the amplitude of the event could indicate different protein conformations. We demonstrated the ability of a functionalized nanopore to measure the recognition and interaction on a single molecule scale. It can provide deeper insight and understanding of the stochastic interaction of various biomolecules.
    URI
    https://doi.org/10.32469/10355/5767
    https://hdl.handle.net/10355/5767
    Degree
    M.S.
    Thesis Department
    Biological engineering (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • 2008 MU theses - Freely available online
    • Biological Engineering electronic theses and dissertations - CAFNR (MU)
    • Biological Engineering electronic theses and dissertations - Engineering (MU)

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