[-] Show simple item record

dc.contributor.advisorHaidekker, Mark A., 1963-eng
dc.contributor.authorMilich, Kacyeng
dc.date.issued2005eng
dc.date.submitted2005 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file viewed on (January 24, 2007)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2005.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] When measuring fluorescence emission intensity, both the dye concentration and the optical absorption properties of the solvent influence the measured intensity. As a consequence, intensity-based assays (for example quenching studies or viscosity measurements using molecular rotors) lack precision. We present a ratiometric fluorophotometer capable of determining fluorescence emission simultaneously with fluid absorption at the dye's excitation and absorption wavelengths. Using suitable models, dye turbidity, emission re-absorption and dye concentration can be determined, and the measured emission intensity corrected. Validation of the prototype showed excellent correlation of measured values with commercial fluorometer (R2=0.99) and spectrometer (R2=0.97). In addition, only instrument constants remain to link emission intensity to quantum yield, a metric which is generally hard to determine. Using the same instrument with only minor modifications, fluorescence measurement through an optical fiber is possible. This allows measurements in remote areas, very small samples, and closed fluid loops. This instrument is characterized by inexpensive design, robust technology based on light-emitting diodes, its flexibility, the reduced footprint due to the integration of two standard instruments, and its increased measurement precision.eng
dc.identifier.merlinb57678613eng
dc.identifier.urihttp://hdl.handle.net/10355/5837
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campuses of the University of Missouri.eng
dc.sourceSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshFluorescence -- Measurementeng
dc.subject.lcshRatiometer (Electric meter)eng
dc.subject.lcshFluorimetereng
dc.titleA ratiometric fluorometer for reduced sensitivity against solvent artifactseng
dc.typeThesiseng
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record