Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2004 Summer Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2004 Summer Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Chromatin-level regulation in maize [abstract]

    Barber, Wesley
    Cone, Karen Camille, 1952-
    View/Open
    [PDF] Chromatin-level regulation in maize.pdf (10.15Kb)
    Date
    2004
    Contributor
    University of Missouri-Columbia. Office of Undergraduate Research
    Format
    Abstract
    Metadata
    [+] Show full item record
    Abstract
    Epigenetic regulation of gene expression is determined by chromatin packing. Transcriptionally active genes are associated with open, less compacted chromatin, whereas transcriptionally less active or silent genes are found in tightly packed, inaccessible chromatin. At the DNA level, genes that are silenced by chromatin frequently have high levels of cytosine methylation. Our goal is to understand the mechanisms behind chromatin-level regulation in maize. We are taking a functional genomics approach by making transgenic lines that use RNA interferences (RNAi) to knock down expression of a large number of chromatin genes and assay the phenotypes in the resulting RNAi lines. Among the genes we are studying are chr101 and chr106, which are duplicates with sequence similarity to a gene in Arabidopsis that is involved in chromatin remodeling and DNA methylation. As a step in understanding how chr101 and chr106 function, we have crossed the RNAi lines to a line containing the Pl-Blotched gene. This gene is an allele of the Purple gene, which controls the synthesis of purple anthocyanin pigments in the corn plant. Pl-Blotched leads to a variegated pattern of pigmentation, rather than the normal uniformly purple phenotype. At the molecular level, this variegation is associated with closed chromatin and high levels of DNA methylation. If the chromatin state of Pl-Blotched is regulated by chr101 and / or chr106, then knocking out expression of these genes by RNAi should lead to higher levels of expression of Pl-Blotched. To test this idea, we are comparing anthocyanin levels in Pl-Blotched plants carrying the chr101 and chr106 RNAi transgenes to the pigment levels in non-transgenic siblings.
    URI
    http://hdl.handle.net/10355/585
    Part of
    2004 Summer Undergraduate Research and Creative Achievements Forum (MU)
    Collections
    • 2004 Summer Undergraduate Research and Creative Achievements Forum (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems