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    • Graduate School - MU Theses and Dissertations (MU)
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    • 2006 Dissertations (MU)
    • 2006 MU dissertations - Access restricted to UM
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    Examination of host genetic determinants of the hypersensitive response in Nicotiana edwardsonii to CaMV and TMV

    Cawly, John D., 1949-
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    Date
    2006
    Format
    Thesis
    Metadata
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    Abstract
    [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The hypersensitive response (HR) is an indicator of genetic resistance when screening for disease resistance to viral pathogens. Genetic resistance has been described as having two characteristics: rapid cell death at the site of inoculation and localization of the pathogen to that site. I examined a resistant Nicotiana hybrid, which does not respond with HR-mediated cell death, yet maintains resistance to Cauliflower mosaic virus (CaMV) strain W260. Another host gene, CCD1, blocks both HR-mediated and systemic cell death induced specifically by P6 of CaMV strain W260. Interfering double stranded RNA (RNAi) technology has proven to be a very effective way to silence a single gene in plants. With the design and construction of gene silencing hairpins homologous with known conserved sequences of identified R genes, it is possible to silence large families of R genes. Examination of the HR in these silenced plants can identify putative resistance genes. With these silencing hairpins, I showed that the endogenous N gene can be silenced with both constructs. Additionally, resistance to another virus, Tomato bushy stunt virus (TBSV), breaks down in the silenced plants. This is evidence that not only the N gene can be silenced, but also that resistance genes that differ from the N gene by as much as 10% can also be silenced when homologous sequences are used.
    URI
    https://doi.org/10.32469/10355/5880
    https://hdl.handle.net/10355/5880
    Degree
    Ph. D.
    Thesis Department
    Plant pathology (MU)
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    Access is limited to the campuses of the University of Missouri.
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    • 2006 MU dissertations - Access restricted to UM
    • Plant Sciences electronic theses and dissertations (MU)

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