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dc.contributor.advisorKirk, Mark D.eng
dc.contributor.authorPierret, Chris, 1972-eng
dc.date.issued2008eng
dc.date.submitted2008 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on August 3, 2009)eng
dc.descriptionVita.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2008.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] We describe a novel neural stem cell (NSC) culture system, derived from embryonic stem (ES) cells, that maintains and expands a population of neural stem cells similar to those in the developing and adult brain. The identity of cellular and molecular components within the cultures was documented using quantitative RT-PCR, immunocytochemistry, neurosphere forming assays, and FLOW cytometry. Furthermore we compare the in vitro neural stem cell niche to neurogenic tissue of the adult brain and to other neural induction protocols and explore the use of 3-D physical scaffolding to better mimic the physical growth environment of living tissue. We show that embryonic stem cells can be induced to form structures that exhibit key properties of a developing neural stem cell niche. We believe this system can serve as a usefull model for studies of neurogenesis and stem cell maintenance in the NSC niche as well as for applications in stem cell transplantation.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.identifier.merlinb7060101xeng
dc.identifier.oclc428980492eng
dc.identifier.urihttps://doi.org/10.32469/10355/6054eng
dc.identifier.urihttps://hdl.handle.net/10355/6054
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campus of the University of Missouri--Columbia.eng
dc.sourceSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshNeural stem cells -- Research -- Social aspects -- Study and teachingeng
dc.titleCharacterization of an in vitro neural stem cell niche with educational component : stem cells and societyeng
dc.typeThesiseng
thesis.degree.disciplineBiological sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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