[-] Show simple item record

dc.contributor.advisorLorson, Christian (Christian Lawrence), 1969-eng
dc.contributor.authorDickson, Alexa Meganeng
dc.date.issued2008eng
dc.date.submitted2008 Springeng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2008.eng
dc.descriptionVita.eng
dc.description"May 2008"eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Spinal Muscular Atrophy (SMA) is a genetic disease that is intrinsically linked to an alternative RNA splicing event that leads a defective protein. To better understand how aberrant RNA processing events occur and lead to disease development, we studied cis and trans elements associated with survival motor neuron (SMN) pre-mRNA splicing. As a means to move more towards a translational approach to restore proper SMN pre-mRNA splicing, we developed a negatively-acting bifunctional RNA that was shown in several different assays to function both in vitro and in the more complex environment of the SMA mouse model in vivo. To further extend our understanding of pre-mRNA processing and the factors involved in its regulation, we utilize the Minute Virus of Mice (MVM) as a model system to study the fundamental process of alternative splicing. We examined the cis elements within the MVM genome that control the expression of an exon by placing the elements in a heterologous context. We have also identified trans elements involved in MVM alternative splicing. Taken together, this research examined the viral life cycle by analyzing the critical steps in alternative splicing involved in a viable MVM infection, and will also give insights into the fundamental biological process of alternative splicing.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.identifier.merlinb6679979xeng
dc.identifier.oclc318898724eng
dc.identifier.urihttps://doi.org/10.32469/10355/6057eng
dc.identifier.urihttps://hdl.handle.net/10355/6057
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campus of the University of Missouri--Columbia.eng
dc.subject.meshMuscular Atrophy, Spinal -- etiologyeng
dc.subject.meshRNA Splicing -- geneticseng
dc.subject.meshSMN Complex Proteins -- geneticseng
dc.subject.meshViral Nonstructural Proteins -- geneticseng
dc.titleAlternative RNA processing and strategies to modulate splicingeng
dc.typeThesiseng
thesis.degree.disciplineMicrobiology (Medicine) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record