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    • Graduate School - MU Theses and Dissertations (MU)
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    • 2008 Dissertations (MU)
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    Pollen-pistil interactions in nicotiana

    Lee, Christopher B., 1973-
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    [PDF] research.pdf (8.112Mb)
    Date
    2008
    Format
    Thesis
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    Abstract
    [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] To screen for potential mates, angiosperm pistils provide a physical and biochemical barrier that promotes the growth of desirable pollen while preventing growth from undesirable pollen. The pistil controls pollination by secreting molecules that affect pollen tube growth into the extra-cellular matrix (ECM) that pollen tubes grow through. In this work, I investigated pollen-pistil interactions in Nicotiana section Alatae and found two undescribed pollination-based hybridization barriers; a physical size mismatch barrier between species with long pistils and pollen from small flowers, and a molecular rejection mechanism in reciprocal crosses. Several arabinogalactan glycoproteins (AGPs) secreted into the ECM directly affect pollen tube growth. For example, the 120 kDa glycoprotein (120K), required for self-incompatibility associates with vacuole membranes in growing tubes. It is unknown how 120K impacts pollen tube growth, or how it traffics to a vacuole. I identified pollen proteins that bind to 120K, and may function in its trafficking. An E3 ubiquitin ligase, SBP1, and a pollen-specific C2-domain-containing protein (NaPCCP) were identified. Protein interaction domains between 120K and SBP1 or NaPCCP were identified. Furthermore, NaPCCP interacts with phosphatidylinositol 3-phosphate (PI3P). PI3P is associated with endosomes, therefore, NaPCCP may function in an endocytic route of 120K and associated proteins.
    URI
    https://doi.org/10.32469/10355/6089
    https://hdl.handle.net/10355/6089
    Degree
    Ph. D.
    Thesis Department
    Biological sciences (MU)
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    Access is limited to the campuses of the University of Missouri.
    Collections
    • 2008 MU dissertations - Access restricted to UM
    • Biological Sciences electronic theses and disserations (MU)

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