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dc.contributor.advisorBowles, Douglas K. (Douglas Kent), 1962-eng
dc.contributor.authorFleenor, Bradley S., 1977-eng
dc.date.issued2008eng
dc.date.submitted2008 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionVita.eng
dc.description"August 2008"eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2008.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Veterinary biomedical sciences.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Percutaneous transluminal coronary angioplasty (PTCA) stimulates vascular cell proliferation and migration resulting in restenosis of the revascularized artery. Exercise training (EX) following PTCA reduces stenosis diameter and increases event-free survival. Our aim was to determine the origin of the cells that migrate to form the neointima (NI) and determine if EX inhibits lesion size and alters the NI ECM composition in a porcine PTCA model. Utilizing the bromodeoxyuridine (BrdU) pulsechase technique, it was determined that few (<3%) BrdU positive cells originating from the adventitia occupy the NI. Smooth muscle myosin heavy chain (SMMHC), a SMC differentiation mark revealed uniform staining of NI cells, showing SMC primarily occupy the NI. Furthermore, EX significantly inhibited NI SMC proliferation and lesion size in the left anterior descending (LAD), but not in the left circumflex (LCX) coronary artery. EX also decreased type I collagen in the LAD but not in the LCX, implying this ECM protein participates in SMC proliferation. Total collagen was increased whereas, fibronectin was decreased in both the LAD and LCX with EX. In vitro analysis demonstrated a role for IL-6 and IL-10 to inhibit the up-regulation of collagen type I and fibronectin gene expressions in cultured SMC. Collectively, these data demonstrate a role for SMC to migrate and form the NI; whereas, EX inhibits SMC proliferation decreasing lesion size in the LAD. Furthermore, EX alters the ECM composition of the NI that may be partially mediated via the anti-inflammatory cytokines IL-6 and IL-10.eng
dc.identifier.merlinb66787324eng
dc.identifier.oclc318651478eng
dc.identifier.urihttps://hdl.handle.net/10355/6097
dc.identifier.urihttps://doi.org/10.32469/10355/6097eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campuses of the University of Missouri.eng
dc.subject.lcshAngioplastyeng
dc.subject.lcshCoronary arteries -- Stenosis -- Relapseeng
dc.subject.lcshExercise therapyeng
dc.titlePost-angioplasty restenosis: the effects of exercise trainingeng
dc.typeThesiseng
thesis.degree.disciplineVeterinary biomedical sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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