Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Health Sciences Research Day (MU)
    • 2009 Health Sciences Research Day (MU)
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Health Sciences Research Day (MU)
    • 2009 Health Sciences Research Day (MU)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Brain MS imaging after ischemic stroke in mice : insight for brain injury by abnormal proteolysis [abstract]

    Saeks, Jeffrey
    View/Open
    [PDF] BrainMSImagingIschemic[abstract].pdf (169.8Kb)
    Date
    2010-02
    Contributor
    University of Missouri--Columbia. School of Medicine
    Format
    Abstract
    Metadata
    [+] Show full item record
    Abstract
    Stroke, the 3rd leading cause of death in the US, is caused by blood clotting in the cerebral arteries leading to brain oxygen deprivation and cerebral infarction. To represent the stroke condition, we produced a mouse model of focal cerebral ischemia followed by reperfusion. We then employed matrix-based mass spectrometry imaging to compare the molecular differences between the ischemic versus nonischemic regions and attempted to identify mass signatures that correlate with abnormal brain proteolytic activity and disease. In response to ischemia, the brain showed swelling due to traumatic injury to the brain. The optical image indicates the ischemic area (A, dotted red line). The total averaged spectra shows ion rich features throughout the measured mass range (B). The overall anatomical brain structure was intact evidenced by selective localization of a number of molecular ions (C, colorcoded) while other distinct ions are shown to co-localize well to the area of injury (D). The ions which localized to the area of injury were found in between 5 to 20 kilodaltons (kD) in size. Based on such information, tissues in the ischemic regions were homogenized and molecules were selectively separated by gel electrophoresis and subsequently extracted from the gel. Attempts to adequately enrich these molecules for identification by mass spectrometry are still in process. Identification of these molecules, which are specifically present in the ischemic regions of the brain, may have profound application in the development of novel biomarkers for diagnosis and/or therapies of stroke.
    URI
    http://hdl.handle.net/10355/6191
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • 2009 Health Sciences Research Day (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems