dc.contributor.advisor | Cohn, Leah A. | eng |
dc.contributor.author | DeClue, Amy E. | eng |
dc.date.issued | 2007 | eng |
dc.date.submitted | 2007 Summer | eng |
dc.description | The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. | eng |
dc.description | "August 2007" | eng |
dc.description | Includes bibliographical references. | eng |
dc.description | Thesis (M.S.) University of Missouri-Columbia 2007. | eng |
dc.description | Dissertations, Academic -- University of Missouri--Columbia -- Veterinary medicine and surgery. | eng |
dc.description.abstract | [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Previous studies have shown that ketamine attenuates endotoxin (LPS)-induced production of tumor necrosis factor (TNF)-[alpha] in murine models. Because TNF-[alpha] is a key mediator of the systemic and pulmonary inflammatory response to infection, we hypothesized that ketamine would ameliorate LPS-induced acute lung injury (ALI) in rats and prevent the hemodynamic and immunologic alterations in dogs. Forty male CD rats were divided into 4 treatment groups: control, ketamine, LPS and LPS+ ketamine. Rats were euthanized at either 1 or 6 hours after LPS administration for determination of serum TNF-[alpha] activity or pulmonary evaluation. Ketamine treatment blunted LPSinduced serum TNF-[alpha] activity but did not ameliorate the pulmonary histopathologic changes associated with endotoxemia. For the second study, nine dogs were randomized to either ketamine or placebo treatment groups in a cross-over design. At 30 min, LPS was administered. Heart rate (HR), systolic arterial blood pressure (SAP), plasma TNF-[alpha] activity and white blood cell (WBC) counts were evaluated. Dogs in the ketamine group had significantly lower HR and peak plasma TNF-[alpha] activity after LPS administration versus placebo. There were no differences detected between treatment groups for SAP or white blood cell counts. Based on these data, while ketamine ameliorates TNF-[alpha] production in rats and dogs, it is unlikely to have a clinically important impact on the development of acute lung injury, perturbations in WBC count or SAP during Gram negative sepsis. | eng |
dc.identifier.merlin | b61468484 | eng |
dc.identifier.oclc | 182537868 | eng |
dc.identifier.uri | https://hdl.handle.net/10355/6276 | |
dc.identifier.uri | https://doi.org/10.32469/10355/6276 | eng |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.rights | Access is limited to the campuses of the University of Missouri. | eng |
dc.subject.lcsh | Ketamine -- Physiological effect | eng |
dc.subject.lcsh | Tumor necrosis factor | eng |
dc.subject.lcsh | Rats as laboratory animals | eng |
dc.subject.lcsh | Dogs as laboratory animals | eng |
dc.title | Ketamine immunomodulation during endotoxemia | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Veterinary medicine and surgery (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Masters | eng |
thesis.degree.name | M.S. | eng |