[-] Show simple item record

dc.contributor.authorGu, Chriseng
dc.contributor.corporatenameUniversity of Missouri--Columbia. School of Medicineeng
dc.contributor.meetingnameHealth Sciences Research Day (2010 : University of Missouri)eng
dc.date.issued2010-03eng
dc.description.abstractThis study was aimed at investigating two variables in the photodynamic therapy (PDT) of medulloblastoma cells: light dose and Photofrin concentration. To investigate the effect of Photofrin concentration, a Daoy medulloblastoma cell line was incubated with variable concentrations of Photofrin for 24 hours before exposure to a diode laser (605nm) with a set output of 100 J/cm^2. To investigate the effect of light dose, the same cell line was incubated with 20ug/mL Photofrin for 24 hours before exposure to variable laser outputs. In both investigations, methyl-tetra-zolium (MTT assay; 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) was used to determine cell viability and proliferation after PDT. Standardization of the MTT assay was performed order to accurately compare experimental cell viabilities with known concentrations of the same cell line; the resulting “cell concentration vs. absorbance curve" allows for a standard of comparison of cell viability for cells undergoing PDT.eng
dc.format.extent1 page : illustrationeng
dc.identifier.urihttp://hdl.handle.net/10355/6310
dc.languageEnglisheng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Health Sciences Research Dayeng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.eng
dc.subjectphotodynamic therapyeng
dc.subjectmedulloblastoma cellseng
dc.subject.FASTMedulloblastomaeng
dc.subject.FASTPhotochemotherapyeng
dc.titleIn vitro study of effect of light dose and photofin concentration on photodynamic therapy of medulloblastoma cells [abstract]eng
dc.typeAbstracteng


Files in this item

[PDF]

This item appears in the following Collection(s)

[-] Show simple item record