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dc.contributor.authorHarper, Diane Medvedeng
dc.contributor.authorLankachandra, K. M.eng
dc.contributor.authorWall, Jeffreyeng
dc.contributor.authorJalali, G. R.eng
dc.contributor.authorKershnar, E.eng
dc.contributor.authorHarris, George D.eng
dc.contributor.authorBonham, Aaron Jameseng
dc.contributor.authorShaffer, Todd D.eng
dc.contributor.corporatenameUniversity of Missouri (System)eng
dc.contributor.meetingnameMissouri Life Sciences Summit (2010: University of Missouri--Kansas City)eng
dc.date.issued2010-03eng
dc.descriptionComparative Medicine - OneHealth and Comparative Medicine Poster Sessioneng
dc.description.abstractObjective: 10-17% of women with LSIL cytology truly have CIN 2+ disease at colposcopically directed biopsy and 20% of the CIN 2+ lesions derive from women with LSIL cytology. No molecular marker has yet been able to triage LSIL cytology effectively. If possible, the triage would spare women the referral to colposcopy. Irreversible chromosomal damage occurs during oncogenesis. Increasing cervical dysplastic severity occurs with increasing amplification of the 3q26 chromosomal region. The purpose of this study is to evaluate the test characteristics of 3q26 amplification in women whose routine cytology is reported as LSIL with emphasis on the negative predictive value for reassurance. Methods: We conducted a retrospective study using the available SurePath™ liquid cytology LSIL archival samples from women 17-59 years old which were linked to colposcopically directed biopsy samples taken on average 36 days after cytology sampling (3-90 day range). Nuclei from the LSIL samples were hybridized with a single-copy probe for the chromosome 3q26 region and a control probe for the centromeric alpha repeat sequence of chromosome 7, using standard FISH methods. Amplification was defined as five or more signals present in at least 2 cells. Results: Of the 68 paired cytology/biopsy samples, 3q26 amplification occurred in 40% of the women with CIN 2+ disease (sensitivity 95% CI: 12, 74). There was no amplification in 91% of women with less than CIN 2 disease (specificity 95% CI: 81, 97); and the negative predictive value was 90% (79, 96). Conclusions: The lack of 3q26 amplification in women with screening cytology LSIL results offers reassurance that CIN 2+ disease has not developed. Future prospective studies are ongoing.eng
dc.identifier.urihttp://hdl.handle.net/10355/6349eng
dc.languageEnglisheng
dc.relation.ispartofAbstracts (Missouri Regional Life Sciences Summit 2010)eng
dc.relation.ispartofcommunityUniversity of Missouri System. Missouri Summits. Missouri Regional Life Sciences Summit 2010eng
dc.subjectcancer screeningeng
dc.subjectcolposcopyeng
dc.subjectscreening cytologyeng
dc.subject.lcshColposcopyeng
dc.subject.lcshCytologyeng
dc.title3q26 Amplification is Rarely Present in Women Whose LSIL Cytology does not Represent CIN 2+ Diseaseeng
dc.typePostereng


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