Advancing single molecule fluorescence microscopy through the study of dynamic macromolecules
Metadata[+] Show full item record
We took a two pronged approach to our investigation of protein synthesis by the ribosome: 1) the generation of a novel fluorescence enhancing substrates 2) the development of a method for the generation of fluorescent ribosome constructs. The development and application of fluorescence based diagnostics platforms for the detection of diseases represents a promising field of research. In collaboration with another lab, we established a method for the generation of a fluorescence enhancing substrate that does not require the use of expensive imaging equipment. During further testing of the fluorescence enhancing substrate with biologically relevant samples, our method proved promising for addition applications. Understanding how and why the ribosome synthesizes protein is important to all living organisms. One known challenge for the study of the ribosome has been the generation of complex samples. We developed a process for the generation of ribosomes that is quicker, easier, and cheaper than existing methods. We then applied our methods for the generation of ribosome constructs to investigate how the ribosome, the molecule responsible for protein synthesis, is coordinated during protein synthesis. Our experiments determined that specific interactions play different roles throughout protein synthesis.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.