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dc.contributor.advisorRobertson, John David, 1960-eng
dc.contributor.authorSun, Ruieng
dc.date.issued2009eng
dc.date.submitted2009 Summereng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on Feb 19, 2010).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionThesis advisor: Dr. John D. Robertson.eng
dc.descriptionM.S. University of Missouri--Columbia 2009.eng
dc.description.abstractWhile a number of studies have examined the effect of infections on the trace elements status of mammals, not any similar study in insects are found. In this study, we used inductively coupled plasma-mass spectrometry (ICP-MS) to quantify trace elements of Mg, Mn, Fe, Cu, Zn and Mo levels in the plasma from the tobacco budworm, Heliothis virescens, following infection of the insect with a baculovirus (Helicoverpazea single nucleopolyhedrovirus (HzSNPV)) and bacteria (Micrococcus lysodeikticus) at different times post infection. There are no changes in any metal due to bacterial infection and no differences between any 12 hours post infection mocks versus 12 hours post infection bacterial infected samples. For the larvae with a virus (HzSNPV) treatment, in both fourth and fifth instars, all the trace elements of interest (Mg, Mn, Fe, Cu, Zn, Mo) change over the course of the 72 hours of infection in both instars, which is due to the development of larvae. Iron level in insect plasma with the HzSNPV infection was elevated in the late 4th instar (60 hours post infection) when compared to iron level in the same aged controls. This could be explained by the ability of iron storage protein ferritin to buffer iron, or free iron of damaged tissues leaking intracellular iron into the plasma. Copper level in insect plasma with the HzSNPV infection was lower in the late 4th instar (60 hours post infection) when compared to copper level in the same aged controls, which is probably due to the decline of copper-binding protein prophenoloxidases (PPOs) levels during the course of infection. .eng
dc.description.bibrefIncludes bibliographical references.eng
dc.format.extentvii, 54 pageseng
dc.identifier.oclc539213500eng
dc.identifier.urihttps://doi.org/10.32469/10355/6467eng
dc.identifier.urihttps://hdl.handle.net/10355/6467
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2009 Theseseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subject.lcshTrace elements in the bodyeng
dc.subject.lcshTrace elements -- Pathophysiologyeng
dc.subject.lcshBlood -- Analysiseng
dc.subject.lcshInductively coupled plasma mass spectrometryeng
dc.subject.lcshTobacco budwormeng
dc.subject.lcshInsects -- Viruseseng
dc.titleDetermination of trace elements levels in plasma from larvae in the course of baculoviral and bacterial infections by inductively coupled plasma-mass spectrometry (ICP-MS)eng
dc.typeThesiseng
thesis.degree.disciplineChemistry (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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