dc.contributor.author | Houck, Amy | eng |
dc.contributor.corporatename | University of Missouri--Columbia. School of Medicine | eng |
dc.contributor.meetingname | Health Sciences Research Day (2010 : University of Missouri) | eng |
dc.date.issued | 2010-03 | eng |
dc.description.abstract | Introduction: Arthritis affects a large portion of our population resulting in a significant expense with limited solutions and current treatment has limited options. The current research examines the viability of chondrocytes embedded in an agarose gel using a standard method of viability assessment. Methods: Two groups were created with frozen canine chondrocytes. Group 1 consisted of non-embedded chondrocytes in tissue culture media (n=7). Group 2 consisted of embedded chondrocytes in agarose gel (n=9). Culture medial was collected from the samples for glycosaminoglycan (GAG) content analysis to determine viability of cells. Culture media was collected from Group 1 at days 0, 4, 7, and 10 and from Group 2 at days 0, 3, 5, and 7 for further analysis with GAG assay. GAG content was plotted and compared between groups. Statistical significance was defined as p<0.05. Results: The average GAG content of Group 1 increased from 15.55 [mu]g/[mu]L at day 4, to 27.46 [mu]g/[mu]L at day 7, and 163.07 [mu]g/[mu]L at day 10 showing a continuous increases of GAG in group 1. The average GAG content of Group 2 was 32.03 [mu]g/[mu]L at day 0, 57.23 [mu]g/[mu]L at day 3, 47.92 [mu]g/[mu]L at day 5, and 53.21 [mu]g/[mu]L at day 10. There were no statistically significant differences between collection days in Group 2 (p=0.921). Conclusion: Based on this information in this study, Group 1 non-embedded chondrocytes show viability whereas Group 2 results show that embedded chondrocytes are not a reliable source to grow viable chondrocytes. This study provides information as to the effectiveness of agarose gel as a potential matrix for chondrocytes in bio-engineered tissue. | eng |
dc.format.extent | 1 page | eng |
dc.identifier.uri | http://hdl.handle.net/10355/6505 | |
dc.language | English | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Health Sciences Research Day | eng |
dc.rights | OpenAccess. | eng |
dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. | eng |
dc.subject | arthritis | eng |
dc.subject | chondrocytes | eng |
dc.subject.FAST | Arthritis -- Treatment | eng |
dc.subject.FAST | Cartilage cells | eng |
dc.title | Viability of chondrocytes embedded in agarose gel [abstract] | eng |
dc.type | Abstract | eng |