Understanding genomic evolution and segregation distortion in solanaceae: a COSII linkage map in Nicotiana
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Genetic linkage maps are excellent tools to investigate genomic evolution, genomic structure and quantitative trait loci. In our study, we created comparative linkage maps in a reciprocal cross between Nicotiana longiflora and N. plumbaginifolia. We used genetic markers derived from the Conserved Ortholog Set II (COSII) to investigate synteny between N.longiflora and N. plumbaginifolia (n = 10) and other Solanaceae species (n = 12), such as tomato, pepper and eggplant. We produced two linkage maps; one in a maternal N. longiflora background (43 markers covering 808.1 cM) and a reciprocal map in the maternal N. plumbaginifolia background (54 markers covering 1110.4 cM). Segregation distortion was evident in both backgrounds, especially for the dominant ISSR markers, and was more prevalent in the maternal N. longiflora cross. In both backgrounds, there was a significant preference for the self-fertilizing N. plumbaginifolia allele for distorted markers. Finally, using the COSII markers, we infer many chromosomal rearrangements have occurred since the divergence of Nicotiana and tomato (Solanum lycopersicum) from a common ancestor.