dc.contributor.advisor | JiJi, Ren�e Denise | eng |
dc.contributor.author | Zare, Anahita | eng |
dc.date.issued | 2017 | eng |
dc.date.submitted | 2017 Summer | eng |
dc.description | Field of study: Chemistry. | eng |
dc.description | Dr. Renee D. JiJi, Dissertation Supervisor. | eng |
dc.description | Includes vita. | eng |
dc.description | "July 2017." | eng |
dc.description.abstract | Membrane protein function and structure determination is vital for pharmaceutical development and disease prevention. Despite the various methods of protein structure elucidation in use, a need still exists for techniques that are of adequate resolution, rapid, inexpensive, and compatible with the membrane environment of these proteins. Deep UV resonance Raman (dUVRR) spectroscopy is an emerging structurally sensitive spectroscopic technique for analyzing membrane protein structure. The backbone amide modes are resonance enhanced in dUVRR spectra while the membrane's lipid features are not, resulting in strong membrane protein spectral features in near native environments. In order to better define dUVRR spectral features of membrane proteins, a series of model helical peptides, poly(LA)7, were designed. By substituting select residues of the transmembrane region of poly(LA)7, the propensity of helical structure within a membrane has also been studied. Using these model membrane peptides in increasingly dehydrated environments (aqueous, surfactant, and bilayer), hydration depended changes in the spectra are characterized. | eng |
dc.description.bibref | Includes bibliographical references. | eng |
dc.format.extent | 1 online resource (x, 109 pages) : illustrations (chiefly color) | eng |
dc.identifier.merlin | b129185528 | eng |
dc.identifier.oclc | 1099182665 | eng |
dc.identifier.uri | https://hdl.handle.net/10355/65447 | |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.rights | OpenAccess. | eng |
dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. | eng |
dc.title | Deep UV resonance Raman spectral properties of alpha helical membrane proteins | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Chemistry (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Doctoral | eng |
thesis.degree.name | Ph. D. | eng |