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dc.contributor.advisorBilyeu, Kristin D.eng
dc.contributor.authorBailey, April A.eng
dc.date.issued2009eng
dc.date.submitted2009 Springeng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on October 16, 2009).eng
dc.descriptionThesis advisor: Dr. Kristin D. Bilyeu.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionM.S. University of Missouri--Columbia 2009.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Plant, insect and microbial sciences.eng
dc.description.abstractPlants store inorganic phosphate (Pi) in seeds as phytic acid (PA). Phytic acid chelates essential macro and micronutrients in developing seeds forming phytate, which is nutritionally unavailable to monogastric livestock. Composition changes to lower the PA content of seeds may affect seed viability and seedling performance. This project was conducted in 2006 and 2007 to test the viability, vigor, and field performance of a high phytase activity, transgenic soybean line, CAPPA compared to its progenitor, Jack. CAPPA seeds contain increased phytase activity, a >90% reduction in PA, and a concomitant increase in Pi. A seedling dry mass study measured dry mass gain for each entry from 8 days after planting (DAP) through 21 DAP. A yield component study measured the vegetative and reproductive dry mass gain for each entry from the R5-R8 stages. Laboratory vigor experiments involving standard germination, accelerated aging, cold test, electrical conductivity, and tetrazolium testing were conducted. Field emergence of all entries was low in 2006 and 2007. There was no significant difference in field emergence between CAPPA and Jack in 2006, but in 2007 Jack field emergence was significantly higher than CAPPA. There was no significant difference between CAPPA and Jack in seedling dry mass 8-21 DAP. Jack vegetative dry mass was significantly greater than CAPPA in 2006 and 2007, but CAPPA seeds per plant, seed weight, and yield did not differ significantly from Jack over the two study years. There was no significant difference between Jack and CAPPA standard germination. Germination following accelerated aging was low but did not differ significantly between Jack and CAPPA. Jack seed germination was low following cold testing, but was significantly higher than CAPPA. Tetrazolium testing indicated no significant difference in viability between Jack and CAPPA. Electrolyte leakage of both entries was high, but did not differ significantly. CAPPA vigor appears to be lower than Jack, but due to high variability in the field environment over the study years, field emergence results are not conclusive. Further research is needed to indicate CAPPA's potential as a commercially released low PA soybean line. Emerged CAPPA plants grew to be competitive, high yielding plants.eng
dc.format.extentx, 76 pageseng
dc.identifier.merlin.b71590067eng
dc.identifier.oclc456701161eng
dc.identifier.otherBaileyA-050809-T1116eng
dc.identifier.urihttp://hdl.handle.net/10355/6557eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollection2009 Freely available theses (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2009 Theseseng
dc.subject.lcshPhytic acideng
dc.subject.lcshSoybean -- Seedseng
dc.subject.lcshPlant geneticseng
dc.titleViability, vigor, and field performance of a low phytic acid, high phytase activity soybean line, CAPPAeng
dc.typeThesiseng
thesis.degree.disciplinePlant, insect and microbial sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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