Viability, vigor, and field performance of a low phytic acid, high phytase activity soybean line, CAPPA

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Viability, vigor, and field performance of a low phytic acid, high phytase activity soybean line, CAPPA

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/6557

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dc.contributor.advisor Bilyeu, Kristin D. en_US
dc.contributor.author Bailey, April A. en_US
dc.date.accessioned 2010-03-12T21:06:58Z
dc.date.available 2010-03-12T21:06:58Z
dc.date.issued 2009 en_US
dc.date.submitted 2009 Spring en_US
dc.identifier.other BaileyA-050809-T1116 en_US
dc.identifier.uri http://hdl.handle.net/10355/6557
dc.description The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. en_US
dc.description Title from PDF of title page (University of Missouri--Columbia, viewed on October 16, 2009). en_US
dc.description Thesis advisor: Dr. Kristin D. Bilyeu. en_US
dc.description Includes bibliographical references. en_US
dc.description M.S. University of Missouri--Columbia 2009. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Plant, insect and microbial sciences. en_US
dc.description.abstract Plants store inorganic phosphate (Pi) in seeds as phytic acid (PA). Phytic acid chelates essential macro and micronutrients in developing seeds forming phytate, which is nutritionally unavailable to monogastric livestock. Composition changes to lower the PA content of seeds may affect seed viability and seedling performance. This project was conducted in 2006 and 2007 to test the viability, vigor, and field performance of a high phytase activity, transgenic soybean line, CAPPA compared to its progenitor, Jack. CAPPA seeds contain increased phytase activity, a >90% reduction in PA, and a concomitant increase in Pi. A seedling dry mass study measured dry mass gain for each entry from 8 days after planting (DAP) through 21 DAP. A yield component study measured the vegetative and reproductive dry mass gain for each entry from the R5-R8 stages. Laboratory vigor experiments involving standard germination, accelerated aging, cold test, electrical conductivity, and tetrazolium testing were conducted. Field emergence of all entries was low in 2006 and 2007. There was no significant difference in field emergence between CAPPA and Jack in 2006, but in 2007 Jack field emergence was significantly higher than CAPPA. There was no significant difference between CAPPA and Jack in seedling dry mass 8-21 DAP. Jack vegetative dry mass was significantly greater than CAPPA in 2006 and 2007, but CAPPA seeds per plant, seed weight, and yield did not differ significantly from Jack over the two study years. There was no significant difference between Jack and CAPPA standard germination. Germination following accelerated aging was low but did not differ significantly between Jack and CAPPA. Jack seed germination was low following cold testing, but was significantly higher than CAPPA. Tetrazolium testing indicated no significant difference in viability between Jack and CAPPA. Electrolyte leakage of both entries was high, but did not differ significantly. CAPPA vigor appears to be lower than Jack, but due to high variability in the field environment over the study years, field emergence results are not conclusive. Further research is needed to indicate CAPPA's potential as a commercially released low PA soybean line. Emerged CAPPA plants grew to be competitive, high yielding plants. en_US
dc.format.extent x, 76 pages en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.subject.lcsh Phytic acid en_US
dc.subject.lcsh Soybean -- Seeds en_US
dc.subject.lcsh Plant genetics en_US
dc.title Viability, vigor, and field performance of a low phytic acid, high phytase activity soybean line, CAPPA en_US
dc.type Thesis en_US
thesis.degree.discipline Plant, insect and microbial sciences en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name M.S. en_US
thesis.degree.level Masters en_US
dc.identifier.merlin .b71590067 en_US
dc.identifier.oclc 456701161 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2009 Theses
dc.relation.ispartofcollection 2009 Freely available theses (MU)


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