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dc.contributor.advisorBryda, Elizabeth C.eng
dc.contributor.authorJohnson, Nathan Tylereng
dc.date.issued2014eng
dc.date.submitted2014eng
dc.description.abstractEmbryonic Stem Cells (ESCs) are a critical tool for producing targeted knockout animals and understanding development. ESCs were successfully isolated from rats in 2008 and have been used in producing several targeted knockout animal models. To date, little characterization of rat ESCs (rESCs) has been done. In order to establish a rESC transcriptome, RNA-Seq was done on mRNA from the rESC cell line DAc8, the first male germline competent rat ESC line to be described and the first to be used to generate a knockout rat model. RNA-Seq was chosen as it is currently the most sensitive transcriptome analysis method. In the studies described here, the genes expressed in rat ESCs were identified, and a subset of the undescribed isoforms and unannotated rat genes revealed by this analysis were confirmed by RT-PCR analysis. Importantly, the rESC data allowed comparison with previously reported data for mouse and human ESCs to begin to understand the similarities and differences of the transcriptomes of ESCs from different mammalian species.eng
dc.identifier.urihttps://hdl.handle.net/10355/65943
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by the University of Missouri--Columbia Graduate School.eng
dc.titleTranscriptome profiling of rattus norvegicus embryonic stem cells by RNA-sequencingeng
dc.typeThesiseng
thesis.degree.disciplineVeterinary pathobiology (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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