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dc.contributor.advisorKuehl-Kovarik, M. Cathleeneng
dc.contributor.authorWang, Yong, 1980-eng
dc.date.issued2009eng
dc.date.submitted2009 Summereng
dc.descriptionM.S. University of Missouri-Columbia 2009.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on January 6, 2010).eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionThesis advisor: M. Cathleen Kuehl-Kovarik.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The gonadotropin releasing hormone (GnRH) neuron is the pivotal control center in a tightly regulated reproductive axis. Estradiol typically has a negative effect on GnRH release, but positive feedback occurs to initiate the luteinizing hormone (LH) surge, resulting in ovulation. We performed electrophysiological recordings in GnRH-enhanced green fluorescent protein neurons dissociated from female mice. The results demonstrate that estradiol can inhibit the transient sodium current and decreases peak current density significantly. Apply estradiol directly to the neurons in the dish revealed that: estradiol appears to regulate the sodium current during the action potential in a time-of-day manner. Current densities from estradiol-treated neurons were significantly smaller than control in the morning (1000-1400 hours), but were significantly larger in amplitude than morning currents in the afternoon (1400-1700 hours). Current density in estradiol-treated neurons increased significantly in afternoon cultures, while currents in control cultures remain unchanged. When the current were examined on an hour-by-hour basis, sodium currents in estradiol-treated neurons remained constant in the AM (1000-1200), and then briefly decreased before dramatically increasing between 1300-1400 hours. This suggests that estradiol negatively feeds back on GnRH neurons in the morning, but positively feeds back in the afternoon, which can result a decrease of cell excitability in the morning, but an increase of cell excitability in the afternoon.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.identifier.oclc496011087eng
dc.identifier.urihttps://doi.org/10.32469/10355/6597eng
dc.identifier.urihttps://hdl.handle.net/10355/6597
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2009 Theseseng
dc.rightsAccess is limited to the campus of the University of Missouri--Columbia.eng
dc.subject.lcshEstradiol -- Metabolismeng
dc.subject.lcshCircadian rhythms -- Physiologyeng
dc.subject.lcshLuteinizing hormone releasing hormone -- Physiologyeng
dc.subject.lcshNeurons -- Metabolismeng
dc.subject.lcshSodium channels -- Metabolismeng
dc.subject.meshSodium channels -- Metabolismeng
dc.subject.meshNeurons -- Metabolismeng
dc.subject.meshGonadotropin-Releasing Hormone -- physiologyeng
dc.subject.meshCircadian rhythms -- Physiologyeng
dc.subject.meshEstradiol -- Metabolismeng
dc.titleEstradiol regulates multiple tetrodotoxin-sensitive sodium currents in gonadotropin releasing hormone neurons: implications for cellular regulation of reproductioneng
dc.typeThesiseng
thesis.degree.disciplineVeterinary biomedical sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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