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    Chloroplast GFP expression in tobacco plants agroinfiltrated with tobacco mosaic virus based vectors

    Tah, Tapashree
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    [PDF] research.pdf (1.929Mb)
    Date
    2009
    Format
    Thesis
    Metadata
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    Abstract
    [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Viruses are submicroscopic infectious particles comprising of nucleic acid and protein which can replicate only within living host cells. TMV is a positive single-stranded RNA virus which produces a typical mosaic symptom in infected leaves. TMV has an interesting association with the chloroplast of a plant cell. we have shown that GFP expressed from the TMV vector TMV- 30B-GFP is also found in chloroplasts and we are using TMV-30B-GFP to understand how GFP is expressed inside the chloroplast. Two putative Shine-Dalgarno motifs were found immediately upstream of GFP. To find out the exact role of these motifs, we introduced point mutations in each of them. When the mutation was introduced in both the motifs, GFP expression in the chloroplast was even lesser. Most importantly, the results obtained with p35SGFP show that GFP is somehow able to gain entry to chloroplasts without the aid of any TMV sequences. My work is important because it shows that GFP is an imperfect negative control for the analysis of chloroplast transit sequences.
    URI
    https://doi.org/10.32469/10355/6604
    https://hdl.handle.net/10355/6604
    Degree
    M.S.
    Thesis Department
    Plant sciences (MU)
    Rights
    Access is limited to the campuses of the University of Missouri.
    Collections
    • 2009 MU theses - Access restricted to UM
    • Plant Sciences electronic theses and dissertations (MU)

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