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dc.contributor.advisorSong, Qishengeng
dc.contributor.authorWang, Songjie, 1976-eng
dc.date.issued2008eng
dc.date.submitted2008 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on March 8, 2010).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionDissertation advisor: Dr. Qisheng Song.eng
dc.descriptionVita.eng
dc.descriptionPh. D. University of Missouri--Columbia 2008.eng
dc.description.abstractBursicon is a neurohormone that regulates the cuticle sclerotization and wing expansion process in insects. Bursicon is a heterodimeric cystine knot protein containing two subunits, burs [alpha] and burs [beta], which are encoded by two genes, burs [alpha] and burs [beta]. In the current study, the burs [alpha] and burs [beta] genes were cloned from the house fly Musca domestica. The transcriptional profiles of burs [alpha] and burs [beta] genes were analyzed in the central nervous systems of M. domestica. Recombinant M. domestica bursicon heterodimer (r-bursicon) was expressed in mammalian 293 cells and insect Highfive(TM) cells, and was analyzed for bursicon function. Using the r-bursicon with a fly neck-ligation assay and the DNA microarray analysis, a panel of genes that is regulated by bursicon was identified from M. domestica and also the fruit fly Drosophila melanogaster. Two of these genes, suppressor of hairless and pleckstrin homology, were cloned and sequenced for further study in M. domestica (designated mdSu(H) and mdPH). The mdSu(H) is a transcription factor involved in an important signal transduction pathway, the Notch signaling pathway, and the mdPH is possibly responsible for regulating the G protein coupled receptor 2, which is proposed to be the receptor of bursicon. By studying mdSu(H) and mdPH, we have provided two excellent components that very likely involved in the bursicon regulated signal transduction pathway during the insect cuticle sclerotization and wing expansion process.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxii, 140 pageseng
dc.identifier.oclc549177324eng
dc.identifier.urihttps://hdl.handle.net/10355/6616
dc.identifier.urihttps://doi.org/10.32469/10355/6616eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subject.lcshHousefly -- Geneticseng
dc.subject.lcshInsect hormoneseng
dc.subject.lcshCloningeng
dc.titleCharacterization of the insect cuticle sclerotization hormone bursicon and bursicon-regulated genes in the house fly Musca domesticaeng
dc.typeThesiseng
thesis.degree.disciplinePlant, insect and microbial sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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