Eukaryotic genes in prokaryotic cells

Abstract

"High yields of specific genes or their products can be obtained by cloning the genes (from any organism) as part of arokaryotic plasmid or virus. Furthermore the methods of microbial genetics are available for use in studying the expression of the cloned genes or for specifically modifying them. Molecular Zoning generally involves joining restriction nuclease-generated NA fragments through their "sticky" ends to an appropriate plasid or virus carrier, transformation of Escherichia coli with the recombinant DNA, and verification of the construction by agaroseel electrophoresis. In E. coli, eukaryotic DNA is transcribed and translated. Potential biohazards and suggested guide lines or handling novel recombinant biotypes are discussed.