DNA packaging in isolated bacterial nucleoids : (chromosomes, DNA tertiary structure, nucleoids, DNA folding, DNA supercoiling, nascent RNA, membrane-DNA)
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Properties of nucleoids isolated from Escherichia coli are described. Experiments which investigate the organization of DNA folding and supercoiling in the isolated chromosome are reviewed, and evidence is discussed suggesting a role for certain proteins and nascent RNA molecules in stabilizing the condensed DNA. A model of the conformational organization of the packaged DNA is presented.