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dc.contributor.advisorFan, Xudongeng
dc.contributor.authorGohring, John Thomas, 1984-eng
dc.date.issued2009eng
dc.date.submitted2009 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on March 10, 2010).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionThesis advisor: Dr. Xudong Fan.eng
dc.descriptionM.S. University of Missouri--Columbia 2009.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] We have demonstrated label-free detection of CD4+ and CD8+ T-Lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. For T-Lymphocyte cell detection, white blood cells were isolated from healthy blood and the concentrations adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner capillary surface of the OFRR. Sensing results show excellent capture of CD4+ and CD8+ T-Lymphocytes at medically significant concentrations with a detection time of approximately 30 minutes. Protein biomarkers have recently been heavily researched in their roles in the detection, quantification, and monitoring of aggressive types of breast cancer. We describe a novel, label-free approach for detecting the HER2 extra-cellular domain breast cancer biomarker in human serum samples using the opto-fluidic ring resonator (OFRR). HER2 proteins were spiked in serum at varying concentrations. Results show that the OFRR is able to detect HER2 at medically relevant concentrations in serum ranging from 13 ng/mL to 100 ng/mL in 30 minutes. Our work will lead to a device that can be used as a tool for monitoring disease progression in a low cost sensing setup.eng
dc.description.bibrefIncludes bibliographical references.eng
dc.format.extentvi, 47 pageseng
dc.identifier.oclc558635057eng
dc.identifier.urihttps://doi.org/10.32469/10355/6661eng
dc.identifier.urihttps://hdl.handle.net/10355/6661
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campuses of the University of Missouri.eng
dc.sourceSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshOptofluidicseng
dc.subject.lcshBiosensorseng
dc.subject.lcshT cellseng
dc.subject.lcshCD4 antigeneng
dc.subject.lcshHER-2 proteineng
dc.subject.lcshBiochemical markerseng
dc.subject.lcshBreast -- Cancer -- Prognosiseng
dc.titleDetection of CD4 and CD8 t-lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensoreng
dc.typeThesiseng
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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