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dc.contributor.advisorPrather, Randall S.eng
dc.contributor.authorMordhorst, Bethany Raeeng
dc.date.issued2017eng
dc.date.submitted2017 Falleng
dc.descriptionField of study: Animal sciences.eng
dc.descriptionDr. Randall S. Prather, Dissertation Supervisor.eng
dc.descriptionIncludes vita.eng
dc.description"December 2017."eng
dc.description.abstractGene edited pigs serve as excellent models for biomedicine and agriculture. Currently, the most efficient way to make a reliably-edited transgenic animal is through somatic cell nuclear transfer (SCNT) also known as cloning. This process involves using cells from a donor (which may have been gene edited) that are typically grown in culture and using their nuclear content to reconstruct a new zygote. To do this, the cell may be placed in the perivitelline space of an enucleated oocyte and activated artificially by a calcium-containing media and electrical pulse waves. While it is remarkable that this process works, it is highly inefficient. In pigs the success of transferred embryos becoming live born piglets is only 1-3%. The creation of more cloned pigs enables further study for the benefit of both A) biomedicine in the development of prognosis and treatments and B) agriculture, whether it be for disease resistance, feed efficiency, gas emissions, etc. Two decades of research has not drastically improved the cloning efficiency of most mammals. One of the main impediments to successful cloning is thought to be due to inefficient nuclear reprogramming and remodeling of the donor cell nucleus. In the following chapters we detail our efforts to improve nuclear reprogramming of porcine fetal fibroblasts by altering the metabolism to be more blastomere-like in nature. We used two methods to alter metabolism 1) pharmaceutical agents and 2) hypoxia. After treating donor cells both methods were used in nuclear transfer. Pharmaceutical agents did not improve in vitro development of gestational survival of clones. Hypoxia did improve in vitro development and we are currently awaiting results of gestation.eng
dc.description.bibrefIncludes bibliographical references (pages 187-247).eng
dc.format.extent1 online resource (xx, 248 pages) : illustrations (chiefly color)eng
dc.identifier.merlinb129183428eng
dc.identifier.oclc1098243842eng
dc.identifier.urihttps://hdl.handle.net/10355/66732
dc.identifier.urihttps://doi.org/10.32469/10355/66732eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.eng
dc.titleMetabolic programming of a Warburg effect-like phenotype in donor fibroblasts prior to somatic cell nuclear transfereng
dc.typeThesiseng
thesis.degree.disciplineAnimal sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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