An exploration of mechanisms for Arabidopsis TCP transcription factor activity at an interface of development and immunity
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] "The work presented in this dissertation aimed to characterize new functions and regulatory targets for TCP8, potentially in both defense and development signaling networks. Using in vitro and in vivo promoter interaction screens in yeast and Arabidopsis, respectively, the PTI-related immune receptor gene EFR as well as a set of growth-related BR signaling genes were identified as regulatory targets of TCP8; these findings were verified through direct interaction assays characterization of tcp mutants for associated phenotypes. Additionally, SRFR1-interacting TCPs were shown to be post-translationally modified by SUMO proteins, alongside data suggesting that SRFR1 sequence motifs that facilitate interactions with SUMO are critical to its function. Together, these data describe novel roles for TCP8 and other class I TCPs, as well as novel regulatory mechanisms for their activities in the context of their interactions with SRFR1 and other TPR proteins. As a highly-conserved TF family among plants, including economically relevant crop species, advancing our understanding of TCP regulatory activities could eventually yield translational benefits to agriculture and food production worldwide."--Page 36-37.
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