[-] Show simple item record

dc.contributor.advisorCaldwell, Charles W., M.D.eng
dc.contributor.authorRahmatpanah, Farahnaz B.eng
dc.date.issued2008eng
dc.date.submitted2008 Springeng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on April 1, 2010).eng
dc.descriptionVita.eng
dc.descriptionThesis advisor: Charles W. Caldwell.eng
dc.description"May 2008"eng
dc.descriptionPh. D. University of Missouri-Columbia 2008.eng
dc.description.abstractDNA methylation plays a significant role in cellular differentiation and biologic activity through silencing of gene expression and also appears to be a factor in the progression of different types of cancer, including Small B cell Lymphomas (SBCL). In this work we utilized three different microarray platforms (9K, 12K and 244K) for the genome wide characterization of DNA methylation in Mantle Cell Lymphoma (MCL), Chronic Lymphocytic Leukemia (CLL), and Follicular lymphoma. The DNA methylation analysis revealed differential methylation patterns in SBCLs. Furthermore, the analysis of DNA methylation profiles suggests that the patterns in CLL may not be homogenous. We demonstrated that the heterogeneity in DNA methylation can be explained, at least in a part, by the CD38 expression levels which is a biomarker to predict the prognosis of the disease in CLL patients. We found alterations in DNA methylation of multiple genes, including SFRP2, Groucho/TLEs, DKK1, SOX genes and APC2 that are members of the WNT signaling pathway in CLL patient samples and CLL cell lines. These genes are viewed as WNT antagonists because of their ability to down regulate WNT signaling. Quantitative real time PCR revealed reactivation of these genes in CLL cell lines after treatment with epigenetic modifying drugs which implies the functional impact of DNA methylation and /or histone deacetylase inhibitors on the mRNA expression of these genes.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxix, 184 pageseng
dc.identifier.oclc605046563eng
dc.identifier.urihttps://hdl.handle.net/10355/6863
dc.identifier.urihttps://doi.org/10.32469/10355/6863eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subject.lcshDNA -- Methylationeng
dc.subject.lcshLymphomas -- Genetic aspectseng
dc.subject.lcshGene silencingeng
dc.subject.lcshCD antigens -- Metabolism -- Genetic aspectseng
dc.subject.lcshWnt proteins -- Antagonistseng
dc.titleLarge scale CpG island methylation profiling of small B cell lymphomaeng
dc.typeThesiseng
thesis.degree.disciplineVeterinary pathobiology area program (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record