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dc.contributor.advisorRoberts, R. M. (Robert Michael), 1940-eng
dc.contributor.authorWalker, Angela Marie, 1978-eng
dc.date.issued2008eng
dc.date.submitted2008 Springeng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on April 1, 2010).eng
dc.descriptionIncludes bibliographical referenceseng
dc.descriptionVita.eng
dc.descriptionThesis advisor: R. Michaels Roberts.eng
dc.description"May 2008"eng
dc.descriptionPh. D. University of Missouri-Columbia 2008.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Veterinary pathobiology area program.eng
dc.description.abstractThe Type I interferons (IFN) have major roles in the innate immune response to viruses, a function that is believed to have led to rapid expansion in the number and complexity of their genes. IFNT, which is a unique Type I IFN restricted to pecoran ruminants, also has a specialized role in maternal recognition of pregnancy in cattle. This work has two main aims 1) determine whether male and female blastocysts differ in the kind and number of IFNT they express and whether this pattern changes over development and 2) provide the first comprehensive annotation of the Type I IFN locus in Bos taurus, thereby providing an insight into the functional evolution of the Type IFN in ruminants. Data collected for the first aim indicate that female blastocysts do not transcribe a different set of IFNTs than males (p=0.54). However, significant differences (p < 0.001) were evident among conceptuses of different age, indicating that additional genes may be transcribed as IFNT production increases during development. The data collected for the second aim revealed the Type I IFN locus has undergone significant rearrangement and expansion in bovine compared to mouse and human. The IFNW subfamily is greatly expanded compared to other species, comprising 24 potentially functional genes. Selective pressure analysis found the regulatory regions of the IFNW are diverging faster than the coding regions. The identification of a new Type I IFN subfamily that is expressed from virally challenged bovine kidney cells is the most striking finding of the second aim.eng
dc.format.extentxi, 177 pageseng
dc.identifier.oclc605037785eng
dc.identifier.otherWalkerA-110509-D9687eng
dc.identifier.urihttp://hdl.handle.net/10355/6864eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2008 Freely available dissertations (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2008 Dissertationseng
dc.subject.lcshInterferon -- Metabolism -- Genetic aspectseng
dc.subject.lcshBlastocysteng
dc.subject.lcshCattle -- Geneticseng
dc.subject.lcshGenetic transcriptioneng
dc.subject.lcshInterferon -- Evolutioneng
dc.subject.lcshMolecular evolutioneng
dc.titleThe type I IFN of Bos tauruseng
dc.typeThesiseng
thesis.degree.disciplineVeterinary pathobiology area program (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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